| 转基因杨树对美国白蛾幼虫中肠保护酶系统的影响(英文) |
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| 引用本文： | 丁双阳,孟秀芹,李学锋.转基因杨树对美国白蛾幼虫中肠保护酶系统的影响(英文)[J].林业研究,2001,12(2):119-122. |
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| 作者姓名： | 丁双阳 孟秀芹 李学锋 |
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| 作者单位： | 北京林业大学森林资源与环境学院! 北京100083，辽宁省清原县林业局森保站! 清原113300，中国农业大学应用化学系!北京100094 |
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| 基金项目： | the China Postdoctoral Research Foundation. |
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| 摘 要： | 以转Bt基因欧洲黑杨(P.nigra L.)和转CpTI基因毛白杨(Populus tomentosa)叶片饲喂4-5龄美国白蛾（Hyphantria cunea Drury）幼虫，对其体内保护酶系统活性进行测定。结果表明，饲喂两种转基因杨树叶片的幼虫中肠保护酶表现出相似的变化规律，SOD、CAT和POD三种酶的活性在饲喂后数小时内逐渐增加，某一时刻达到最高值，此后突然下降。饲喂转Bt基因杨树叶片的幼虫，其中肠SOD、CAT活性峰值出现在饲喂后的24小时，POD活性峰值出现在饲喂后的12小时；饲喂转CpTI基因杨树叶片的幼虫，其中肠三种保护酶活性高峰出现时间均较前者滞后12小时。本文还比较了饲喂两种转基因叶片不同中毒程度的美国白蛾幼虫体内保护酶活性，发现不论饲喂那种转基因叶片，中毒较轻者其体内保护酶活性显著高于中毒较重者，这种差异在饲喂CpTI叶片的处理株表现尤为明显。
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| 关 键 词： | 转基因杨树 美国白蛾 保护酶 |
| 收稿时间： | 26 December 2000 |
Effects of two kinds of transgenic poplar on protective enzymes system in the midgut of larvae of American white moth |
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| Ding Shuang-yang,Li Huai-ye,Li Xue-feng,Zhang Zhi-yi.Effects of two kinds of transgenic poplar on protective enzymes system in the midgut of larvae of American white moth[J].Journal of Forestry Research,2001,12(2):119-122. |
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| Authors: | Ding Shuang-yang Li Huai-ye Li Xue-feng Zhang Zhi-yi |
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| Institution: | (1) College of Forestry Resources and Environment, Beijing Forestry University, 100083 Beijing, P. R. China;(2) Forest Pest Management Station, Forestry Bureau of Qingyuan County, 113300 Liaoning Province, P. R. China;(3) Department of Applied Chemistry, China Agriculture University, 100094 Beijing, P. R. China;(4) College of Forestry Resources and Environment, Beijing Forestry University, 100083 Beijing, P. R. China |
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| Abstract: | The leaves of Bt (Bacillus thuringiensis) transgenic poplar (Populus nigra L.) and CpTI (Cowpea trypsin inhibitor) transgenic poplar ((P. tomentosa×P. bolleana)×P. Tomentosa) were taken to feed the 4th-5th-instar larvae of American white moth (Hyphantria cunea (Drury)) for determination of the activities of the protective enzyme system inside larvae's body. The physiological and
biochemical effects of the transgenic poplars on the larvae were studied. The results showed that the two kinds of transgenic
poplars had similar effects on the protective enzyme system in the midgut of larvae. The activities of superoxide dismutase,
catalase, and peroxidase in midgut of the larvae increased gradually, reached the highest value at a certain time, and then
decreased suddenly. For the larvae that were fed with the leaves of Bt transgenic poplar, the peak value of superoxide dismutase
and catalase presented at the time of 24-h feeding, while the peak of peroxidase took place at the time of 12-h feeding. The
activities of these protective enzymes for the larvae that were fed with leaves of CpTI transgenic poplar peaked 12 h later
than that of those fed with leaves of Bt transgenic poplar. The comparison of activities of the protective enzymes was also
carried out between the larvae with different levels of intoxication. It was found that the activities of protective enzyme
of the seriously intoxicant larvae were higher than that of the lightly intoxicant larvae. This difference was more obvious
in the group treated with CpTI transgenic poplar.
Foundation item: This paper was supported by the China Postdoctoral Research Foundation.
Biography: DING Shuang-yang (1966-), female, College of Forestry Resources and Environment, Beijing Forestry University, Beijing 100083
Responsible editor: Chai Ruihai |
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| Keywords: | Transgenic poplar American white moth Protective enzyme system |
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