| 不同种泡桐叶片愈伤组织诱导及其植株再生(英文) |
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| 引用本文： | 范国强,翟晓巧,翟翠娟,毕会涛.不同种泡桐叶片愈伤组织诱导及其植株再生(英文)[J].林业研究,2001(4). |
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| 作者姓名： | 范国强 翟晓巧 翟翠娟 毕会涛 |
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| 作者单位： | 河南农业大学泡桐研究所 郑州450002
(范国强，翟晓巧，翟翠娟)，河南农业大学泡桐研究所 郑州450002(毕会涛) |
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| 基金项目： | This paper was supported by National Nature Science Foundation of China (No. 39870631) and Nature Science Foundation of Henan Province (No. 994011100). |
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| 摘 要： | 在确定5种泡桐叶片诱导愈伤组织基本培养基为MS的基础上,通过不同的NAA和BA的组合,筛选出了毛泡桐(Paulownia tomentosa)、南方泡桐(Pauiownia australis)、白花泡桐(Paulownia fortunei)、兰考泡桐(Paulownia elongata)和豫杂一号泡桐(P.tomentosa x P. fortunei)叶片愈伤组织诱导、芽分化和根分化的最适培养基。MS+0.5NAA+4BA、MS+0.3NAA+2BA、MS+0.5NAA+4BA、MS+0.3NAA+6BA和MS+0.3NAA+8BA分别为五种泡桐树种叶片愈伤组织诱导的最适培养基,上述树种的叶片愈伤组织诱导芽分化最适培养基分别为MS+0.3NAA+12 BA、MS+0.3NAA+12 BA、MS+0.5NAA+12 BA、MS+0.5NAA+12 BA和MS+0.7NAA+12 BA,最后找出了5种泡桐芽诱导根的最适培养基分别为1/2MS+0.1NAA、1/2MS+0.1NAA、1/2MS、1/2MS+0.3NAA和1/2MS+0.5NAA。这些结果为开展泡桐基因工程研究和利用不同种泡桐叶片原生质体融合培育泡桐新品种提供了参考。
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| 关 键 词： | 泡桐 愈伤组织诱导 再生植株 培养基 激素 |
Callus induction from leaves of different paulownia species and its plantlet regeneration |
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| FAN Guo-qiang,ZHAI Xiao-qiao,ZHAI Cui-juan,BI Hui-tao.Callus induction from leaves of different paulownia species and its plantlet regeneration[J].Journal of Forestry Research,2001(4). |
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| Authors: | FAN Guo-qiang ZHAI Xiao-qiao ZHAI Cui-juan BI Hui-tao |
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| Abstract: | The experiment was carried out on five different species of Paulownia for callus induction from leaves. MS medium was adopted as basic medium, and from different combinations of NAA and BA the suitable media were determined for callus induction, bud differentiation, and root differentiation of five different species. MS+0.5NAA+4BA, MS+0.3NAA+2BA, MS+0.5NAA+4BA, MS+0.3NAA+6BA, and MS+0.3NAA+8BA were suitable media of callus inductions of leaves, respectively, for Paulownia tomentosa, Paulownia australis, Paulownia fortunei, Paulownia elongata and P. tmentosa x P. fortunei, and MS+0.3NAA+12BA, MS+0.3NAA+12BA, MS+0.5NAA+12BA, MS+0.5NAA+12BA, and MS+0.7NAA+12BA were suitable media for bud differentiation from leaf callus respectively for above five species. The rooting media was determined as 2MS+0.1NAA, 1/2MS+0.1NAA, 1/2MS, 1/2MS+0.3NAA, and 1/2MS+0.5NAA. These results provide reference data for breeding new fine va-rieties with different kinds of Paulownia protoplasts fusions. |
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| Keywords: | Paulownia Callus induction Plantlet regeneration Medium Hormone |
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