| 褐飞虱看家基因启动子克隆与序列分析 |
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| 引用本文： | 彭雷,赵艳,马银花.褐飞虱看家基因启动子克隆与序列分析[J].贵州农业科学,2016(9):4-7. |
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| 作者姓名： | 彭雷 赵艳 马银花 |
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| 作者单位： | 1. 贵州师范大学生命科学学院,贵州贵阳,550001;2. 武汉大学生命科学学院,湖北武汉,430072 |
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| 基金项目： | 国家自然科学基金项目“水稻抗褐飞虱基因多样性持续控制褐飞虱的分子机理”(31230060) |
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| 摘 要： | 为获得组成型表达启动子,以褐飞虱的看家基因肌动蛋白(Acting1)与甘油醛-3-磷酸脱氢酶基因(GAPDH)为研究对象,以Genbank上褐飞虱Actin1mRNA序列设计嵌套引物,通过灰飞虱GAPDH mRNA序列搜寻褐飞虱EST数据库中的同源序列设计嵌套引物,并以设计的嵌套引物通过Tail-PCR技术对褐飞虱Actin 1与GAPDH基因ATG前侧翼序列进行扩增。结果表明:通过染色体步移的Tail-PCR技术获得Actin1与GAPDH基因ATG前侧翼序列,长度分别为2 878bp和1 196bp。BDGP数据库与PLACE软件在线分析获得核心启动子序列及TATA框、CAAT框和GATA框元件。
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| 关 键 词： | 褐飞虱 Actin 1 GAPDH 启动子 Tail-PCR |
Cloning and Sequence Analysis of Brown Planthopper House-keeping Gene Promoter |
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| Abstract: | The ATG front flanking sequence of Nilaparvata lugens Acting 1 and Laodelphax striatella GAPDH was amplified by Tail-PCR from designed nested primers based on nested primers designed from Nilaparvata lugens Actin 1 mRNA sequence in Genbank and the nested primers designed from the homologous sequence in Nilaparvata lugens EST database by searching Laodelphax striatella GAPDH mRNA sequence.Results:The length of ATG front flanking sequence of Acting 1 and GAPDH is 2 878 bp and 1 196 bp respectively.The core promoter sequence,TATA box,CAAT box and GATA box are obtained by on-line analysis of BDGP database and PLACE software. |
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| Keywords: | brown planthopper Actin 1 GAPDH promoter Tail-PCR |
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