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| 非生物胁迫诱导的玉米蛋白激酶基因ZmCIPK1的cDNA克隆和表达分析 | |
| 引用本文： | 边鸣镝,吴忠义,赵久然,王永勤,张秀海,黄丛林,曹鸣庆.非生物胁迫诱导的玉米蛋白激酶基因ZmCIPK1的cDNA克隆和表达分析[J].农业生物技术学报,2008,16(6). |
| 作者姓名： | 边鸣镝 吴忠义 赵久然 王永勤 张秀海 黄丛林 曹鸣庆 |
| 作者单位： | 1. 首都师范大学生命科学院,北京,100037;北京市农林科学院北京农业生物技术研究中心,北京,100097 2. 北京市农林科学院北京农业生物技术研究中心,北京,100097 3. 北京市农林科学院玉米研究中心,北京,100097 |
| 基金项目： | 国家重点基础研究发展规划(973计划)，国家自然科学基金，北京市科委玉米育种创新平台项目 |
| 摘 要： | CIPK（CBL-interacting Protein Kinase）在植物非生物胁迫信号转导中起重要作用。应用电子克隆的方法在玉米中克隆了一个cDNA全长为1395bp的蛋白激酶基因ZmCIPK1（GenBank接受号为EF158034）。其编码的蛋白含有465个氨基酸，分子量为51.98KD，等电点为6.92。推断的ZmCIPK1催化结构域中包含蛋白激酶催化结构域保守的11个亚结构域， 蛋白C端具有植物CIPK蛋白家族特有的包含24个氨基酸的NAF结构域。半定量RT-PCR结果表明，在甘露醇、氯化钠、ABA和4°C低温诱导时，ZmCIPK1在玉米幼苗叶片中表达量明显上调，在24小时内保持较高的转录水平。试验结果表明，ZmCIPK1是一个新的玉米蛋白激酶基因，响应多种非生物胁迫信号。 |
| 关 键 词： | 玉米 类钙调磷酸酶B互作蛋白激酶(CIPK) 非生物胁迫 |
| 收稿时间： | 2008-1-3 |
| 修稿时间： | 2008-6-26 |
cDNA Cloning of Maize Protein Kinase Gene ZmCIPK1 in Response to Abiotic Stress and Its Expression Analysis in Maize |
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| BIAN Ming-di,WU Zhong-yi,ZHAO Jiu-ran,WANG Yong-qin,ZHANG Xiu-hai,HUANG Cong-lin,CAO Ming-qing.cDNA Cloning of Maize Protein Kinase Gene ZmCIPK1 in Response to Abiotic Stress and Its Expression Analysis in Maize[J].Journal of Agricultural Biotechnology,2008,16(6). | |
| Authors: | BIAN Ming-di WU Zhong-yi ZHAO Jiu-ran WANG Yong-qin ZHANG Xiu-hai HUANG Cong-lin CAO Ming-qing |
| Abstract: | Abstract: Protein kinase plays an important role in stress signal transduction in plant. One full-length cDNA encoding a CIPKs homologue was isolated from maize through in silico cloning and named as ZmCIPK1. The predicted ZmCIPK1 protein has 465 amino acids with an estimated molecular mass of 51.98 KD and an isoelectric point of 6.92. The putative kinase catalytic domain of ZmCIPK1 contains the 11 subdomains that are typical in protein kinase. The conserved 24-amino acid motif within the C-terminal non-kinase region of the CIPKs is also found in ZmCIPK1. RT-PCR analysis indicated that ZmCIPK1 expression was up-regulated obviously by mannitol, salt, ABA and low-temperature in young leaves and kept high level during 24 hours. These results demonstrate that ZmCIPK1 is a novel CIPK gene and responds to multiple abiotic signals in maize. key words: maize; CIPK; abiotic stress |
| Keywords: | maize calcineurin B-like protein interacting protein kinase (CIPK) abiotic stress |
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