| 蓝桉树木园异型杂交及花粉污染的微卫星分子标记研究 |
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| 引用本文： | 饶红欣,Réne Vaillancourt,Réne Vaillancourt.蓝桉树木园异型杂交及花粉污染的微卫星分子标记研究[J].林业研究,2008,19(2):136-140. |
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| 作者姓名： | 饶红欣 Réne Vaillancourt Réne Vaillancourt |
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| 作者单位： | RAO Hong-xin（Hunan Provincial Forest Botanic Garden, Changsha 410116, P. R. China）
Briony Patterson（School of Plant Science and Cooperative Research Centre for Forestry, University of Tasmania, Private Bag 55, HOBART 7001,Tasmania, Australia）
Brad Potts（School of Plant Science and Cooperative Research Centre for Forestry, University of Tasmania, Private Bag 55, HOBART 7001,Tasmania, Australia）
Réne Vaillancourt（School of Plant Science and Cooperative Research Centre for Forestry, University of Tasmania, Private Bag 55, HOBART 7001,Tasmania, Australia） |
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| 基金项目： | This study was supported by State Administration of Foreign Experts Affairs, P. R.China (No.2001430007). |
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| 摘 要： | 本文应用4对微卫星分子多态引物,对利用优株嫁接建立的蓝桉(E.globulus)树木园中的118个自由授粉子代的DNA微卫星指纹进行了分析.结果表明:选用的引物具有高度多态性,各个位点扩增得到的平均等位基因数为14.25,树木园中母株的异型杂交率为15%(95%,整体异型杂交率为47.9%,外源花粉污染率为17.6%.由于该树木园的异型杂交率低,其自由授粉的种子应用价值不高,因此应采用大规模人工授粉来制种或选用树木园中异型杂交率高的母株采种.
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| 关 键 词： | 蓝桉 育种园 微卫星 异型杂交率 花粉污染 |
| 文章编号： | 1007-662X(2008)02-0136-05 |
| 收稿时间： | 19 May 2009 |
| 修稿时间： | 2008年3月7日 |
A microsatellite study on outcrossing rates and contamination in an <Emphasis Type="Italic">Eucalyptus globulus</Emphasis> breeding arboretum |
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| Hong-xin Rao,Briony Patterson,Brad Potts,Réne Vaillancourt.A microsatellite study on outcrossing rates and contamination in an Eucalyptus globulus breeding arboretum[J].Journal of Forestry Research,2008,19(2):136-140. |
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| Authors: | Hong-xin Rao Briony Patterson Brad Potts Réne Vaillancourt |
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| Institution: | (1) Hunan Provincial Forest Botanic Garden, Changsha, 410116, P. R. China;(2) School of Plant Science and Cooperative Research Centre for Forestry, University of Tasmania, Private Bag 55, HOBART, 7001, Tasmania, Australia |
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| Abstract: | Four pairs of microsatellite molecular polymorphism primers were used to analyse microsatellite fingerprints of 188 seedlings derived from an open-pollinated progeny grafted Eucalyptus globulus breeding arboretum in Victoria, south-eastern Australia.The microsatellite loci chosen for this study were highly polymorphic with the mean number of alleles per locus of 14.25. Individual mothers varied in their outcrosssing rate estimate from 15% to 95%, the overall outcrossing level in the arboretum was 47.9% and the contamination rate was 17.6%. The high selfing level was likely to result in marked inbreeding depression in the performance of open-pollinated seed lots.Open-pollinated seeds collected from such arboreta are not advisable because of its low genetic quality, although such arboreta may be useful for the seed production through large-scale manual pollination or collecting seeds only from trees or genotypes within the arboretum that have high outcrossing rates. |
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| Keywords: | E globulus breeding arboretum microsatellite outcrossing rate pollen contamination |
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