| 紫花苜蓿高频植株再生体系的建立 |
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| 引用本文： | 钱瑾,刘发央,谢小冬,贾彪.紫花苜蓿高频植株再生体系的建立[J].甘肃农业大学学报,2007,42(1):77-81. |
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| 作者姓名： | 钱瑾 刘发央 谢小冬 贾彪 |
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| 作者单位： | 1. 甘肃农业大学草业学院,甘肃,兰州,730070
2. 兰州大学生命科学院,甘肃,兰州,730000 |
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| 摘 要： | 研究了不同品种间愈伤组织诱导及植株再生的差异.结果表明,MS+2,4-D(2.0 mg/L)+6-BA(0.5mg/L)+蔗糖0.3%+琼脂0.08%,MS+KT(0.5 mg/L)+NAA(0.01 mg/L)+蔗糖0.3%+琼脂0.08%,1/2MS+NAA(0.01 mg/L)+蔗糖0.15%+琼脂0.08%分别是紫花苜蓿的愈伤组织诱导、芽、根分化的适宜诱导培养基.
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| 关 键 词： | 紫花苜蓿 子叶节 下胚轴 愈伤组织 植株再生 |
| 文章编号： | 1003-4315（2007）01-0077-05 |
| 修稿时间： | 2006-06-01 |
Establishment of high frequency regeneration system in Alfalfa(Medicago sativa L.) |
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| QIAN Jin,LIU Fa-yang,XIE Xiao-dong,JIA Biao.Establishment of high frequency regeneration system in Alfalfa(Medicago sativa L.)[J].Journal of Gansu Agricultural University,2007,42(1):77-81. |
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| Authors: | QIAN Jin LIU Fa-yang XIE Xiao-dong JIA Biao |
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| Institution: | 1. College of Prataculture,Gansu Agricultural University,Lanzhou 730070; 2. College of Life Sciences,Lanzhou University,Lanzhou 730000,China |
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| Abstract: | This paper is to probe into the difference of callus induction and plants regeneration among different alfalfa cultivars.The results showed that the most suitable for restoring the tissue,bud and root were as follows:MS 2,4-D(2.0 mg/L) 6-BA(0.5 mg/L) sucrose 0.3 % agar 0.08 %,MS KT(0.5 mg/L) NAA(0.01 mg/L) sucrose 0.3 % agar 0.08 %,1/2MS NAA(0.01 mg/L) sugar 0.15 % agar 0.08 % separately. |
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| Keywords: | Medicago sativa L -hypocoty1-hypocotyls-callus-plant regeneration |
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