| 小麦-黑麦1BL/1RS易位系99/2439中蛋白激酶基因的克隆 |
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| 引用本文： | 王祖华,牛吉山.小麦-黑麦1BL/1RS易位系99/2439中蛋白激酶基因的克隆[J].安徽农业科学,2008,36(14):5823-5825. |
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| 作者姓名： | 王祖华 牛吉山 |
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| 作者单位： | 洛阳理工学院环境与化学系,河南洛阳,471023;河南农业大学国家小麦工程技术研究中心,河南郑州,450002 |
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| 基金项目： | 河南省杰出青年科学基金资助(021000800). |
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| 摘 要： | 目的]克隆和分析小麦受体蛋白激酶基因片段。方法]对经白粉菌诱导24 h的小麦-黑麦1BL/1RS易位系99/2439叶片中cDNA进行5′-RACE,获得小麦受体蛋白激酶基因的部分片段,并分析其蛋白质序列同源性。结果]通过RACE获得了长度为1 708bp的小麦受体蛋白激酶基因片段。该片段的氨基酸序列(S1125)与基因Lrk19在641个氨基酸跨度内有86%相同,91%相似。S1125与小麦抗锈病基因Lrk10在636个氨基酸跨度内有84%相同,88%相似。S1125可能为丝氨酸-苏氨酸激酶。结论]所克隆的小麦蛋白激酶基因片段与Lrk19基因和小麦抗锈病基因Lrk10具有较高的同源性。
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| 关 键 词： | 小麦-黑麦1BL/1RS易位系99/2439 RACE 克隆 白粉病 |
| 文章编号： | 0517-6611(2008)14-05823-03 |
| 修稿时间： | 2008年3月10日 |
Cloning of Protein Kinase Gene in Wheat-rye 1BL/1RS Translocation Line 99/2439 |
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| WANG Zu-hua.Cloning of Protein Kinase Gene in Wheat-rye 1BL/1RS Translocation Line 99/2439[J].Journal of Anhui Agricultural Sciences,2008,36(14):5823-5825. |
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| Authors: | WANG Zu-hua |
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| Abstract: | Objective] The research aimed to clone and analyze the fragment of protein kinase gene in wheat receptor.Method] 5′-RACE wasmade on cDNAin the leaves ofwheat-rye 1BL/1RStranslocation line 99/2439 after beinginduced bypowderymildewfor 24 h toobtain some partofprotein kinase gene in wheat receptor.And the homologyofits protein sequence was analyzed.Result]Through RACE,the fragment of proteinkinase gene in wheat receptor with the length of1 708 bp was obtained.The aminoacid sequence(S1125)ofthis fragment had 86% homologyand91% similarity with gene Lrk19 in the span of 641 amino acids.S1125 had 84% homology and 88% similarity with rust resistance gene Lrk10 inthe span of 636 amino acids.S1125 was probably serine- threonine protein kinase.Conclusion] The cloned fragment of protein kinase gene inwheat receptor had higher homologywith Gene Lrk19 and rust resistance gene Lrk10 in wheat. |
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| Keywords: | Wheat-rye 1BL/1RStranslocation line 22/2439 RACE Cloning Powderymildew |
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