| 不同氮素形态配比对饲料桑幼苗叶片叶绿素荧光特性和能量分配的影响 |
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| 引用本文： | 许 楠,钟海秀,沙 威,伍一宁,张会慧,孙广玉.不同氮素形态配比对饲料桑幼苗叶片叶绿素荧光特性和能量分配的影响[J].中国农学通报,2015,31(26):18-25. |
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| 作者姓名： | 许 楠 钟海秀 沙 威 伍一宁 张会慧 孙广玉 |
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| 作者单位： | (;1.黑龙江省科学院自然与生态研究所,哈尔滨 150040；;2.湿地与生态保育国家地方联合工程实验室,哈尔滨 150040；;3.黑龙江省湿地与恢复生态学重点实验室,哈尔滨 150040；;4.东北林业大学生命科学学院,哈尔滨 150040） |
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| 基金项目： | 基金项目：国家科技支撑项目(2011BAD08B02-3)；黑龙江省自然科学基金重点项目(ZD201105)。 |
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| 摘 要： | 为研究饲料桑‘青龙’幼苗叶片光合电子传递和分配对NH4+-N和NO3--N不同形态氮配比的响应，以不同氮素形态配比为处理手段，通过测定饲料桑‘青龙’幼苗叶片的净光合速率响应曲线、以及荧光参数来测算光合电子传递速率和分配去向。结果表明，正常大气CO2浓度380 μmol/(m2·s)]处理下，饲料桑‘青龙’幼苗叶片较多的激发能以热量的形式耗散，n(NH4+-N):n(NO3--N)为75:25至50:50时可使更多的激发能向光化学反应方向的分配，降低光合能量的热耗散速率，NO3--N浓度增加使激发能以热量的形式耗散增加；n(NH4+-N):n(NO3--N)为100:0时饲料桑‘青龙’幼苗叶片参与光呼吸的非环式电子流速率(J0)最高，随着n(NH4+-N):n(NO3--N)为75:25时饲料桑‘青龙’幼苗叶片参与碳同化的非环式电子流速率Jc也升至最高。n(NH4+-N):n(NO3--N)为50:50时激发能分配不平衡性系数(β/α-1)下降至最低，激发能分配系数达到平衡。荧光量子产额和热耗散的量子产额(Фf，D) n(NH4+-N):n(NO3--N)为50:50至25:75范围时用于光化学反应的量子产额(ФPSⅡ)所占比例最高，依赖于类囊体膜两侧质子梯度和叶黄素循环的量子产额(ФNPQ)所占比例最低。该结果为探讨饲料桑‘青龙’幼苗光合作用电子流流向和激发能分配对不同氮素形态配比的响应规律，为饲料桑栽培中进行合理的苗期氮肥实施提供基础数据。
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| 关 键 词： | 兔眼蓝浆果 兔眼蓝浆果 嫩枝扦插 内源激素 不定根 IBA |
| 收稿时间： | 2015/4/16 0:00:00 |
| 修稿时间： | 2015/4/27 0:00:00 |
Different N Forms on Energy Allocation in Photosystem of Mulberry Seedlings Leaves |
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| Institution: | (;1.Institute of Nature Resource, Heilongjiang Academy of Sciences, Harbin 150040;2.National and Provincial Joint Engineering Laboratory of Wetland and Ecological Conservation, Harbin 150040;3.Key Laboratory for Wetland and Restoration Ecology in Heilongjiang Province, Harbin 150040;4.College of Life Science, Northeast Forest University, Harbin 150040) |
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| Abstract: | Using forage mulberry (Morus alba L.) variety ‘Qinglong’ as experimental material, effects of nitrate and ammonium nitrogen proportion on Pn-PAR response curve and chlorophyll fluorescence parameters and electrons dissipation of mulberry seedlings cultured in hydroponics were investigated under the same nitrogen amount. The results showed that under normal atmospheric CO2 concentration, some energy dissipated of mulberry seedlings by heat dissipation, when the NH4 -N:NO3--N was at 75:25 to 50:50, more energy was taken advantage by photochemical, and NO3--N increase led to the heat dissipation rise obviously. When NH4 -N:NO3--N was at 100:0, the value of J0 reached the top, and then the value of Jc got up to the top when NH4 -N:NO3--N was at 75:25. The distribution of excitation energy imbalance coefficient (β/α-1) declined to the minimum, while the distribution of excitation coefficient reached the balance. The (ФPSⅡ) got up to the top when the NH4 -N:NO3--N changed from 50:50 to 25:75, and the (ФNPQ) was at the minimum value. The study could provide the basis for appropriate nitrogen application in mulberry cultivation. |
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