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黄色马蹄莲的组织培养研究
引用本文:陆方方,邵果园,陈晓梅.黄色马蹄莲的组织培养研究[J].热带农业科技,2007,30(3):41-42,47.
作者姓名:陆方方  邵果园  陈晓梅
作者单位:西南大学,园艺园林学院,重庆,400716
摘    要:以黄色马蹄莲块茎为外植体组培试验结果:最适合的灭菌时间为0.1%的HgCl2消毒15min;在MS添加2.0mg·L-16-BA+0.1mg·L-1 NAA的培养基上可以很好的诱导产生愈伤组织,并且从愈伤产生丛生芽的数量也最多;在MS添加1.0mg·L-1 6-BA+0.2mg·L-1 NAA的培养基上经过初代培养的芽丛可以健壮生长同时继续增殖出新的愈伤组织;在不添加任何激素的MS培养基上马蹄莲生根率均可以达到90%以上,且试管苗根系良好,生长健壮。

关 键 词:黄色马蹄莲  组织培养  愈伤组织
文章编号:1672-450X(2007)03-0041-02
修稿时间:2007-06-22

Study on Tissue Culture of Zantedeschia ellictiana
LU Fang-fang,SHAO Guo-yuan,CHEN Xiao-mei.Study on Tissue Culture of Zantedeschia ellictiana[J].Tropical Agricultural Science & Technology,2007,30(3):41-42,47.
Authors:LU Fang-fang  SHAO Guo-yuan  CHEN Xiao-mei
Institution:College of Gardening and Horticulture, South-west University, Chongqin 400716, China
Abstract:In this experiment,bulbs of caly lily’s yellow varieties-Zantedeschia ellictiana Engler,was used as explants.The results show that the best disinfectant time by 0.1%HgCl2 was 15 min.The best medium for calli induction was MS + BA2.0mg/L 6 + NAA0.1mg/L with more cluster buds.Buds in the medium of MS + 1.0mg/L 6-BA+0.2mg/L NAA can grow well with new callus proliferation.In the MS medium without any hormone,rooting rate exceeds 90%,and the root grows very well.
Keywords:Zantedeschia ellictiana  tissue culture  calli
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