| Vaccination with pcDNA3-15／60 Naked DNA Encoding the Surface Proteinof Sporozoites in Cryptosporidium parvum |
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| 作者姓名： | HEHong-xuan ZHANGXi-chen YINJi-gang LIJian-hua YANGJu |
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| 作者单位： | DepartmentofVeterinaryPreventiveMedicine,FacultyofAnimalScience,theQuartermasteruniversityofChinesePeople＇sLiberationArmy,Changchun130062,P.R.China |
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| 摘 要： | The CP15/60 gene encoding the CP15/60 surface protein of sporozoites in Cryptosporidium parvum was obtained by PCR so as to research the nucleic vaccine against C.parvum. The eukaryotic expressing vector pcDNA3-15/60 was constructed by inserting CP15/60 gene into pcDNA3 ( ) in Xho Ⅰ and EcoR Ⅰ. A vaccination protocol was the adult pregnant goats inoculated intranasally with the pcDNA3-15/60 plasmid and their offspring were infected with C.parvum oocysts. The results showed that the pcDNA3-15/60 plasmid can induce the immune response of goats and the vaccinated goats can transfer the immunity to offspring conferring protection against C.parvum infection. These suggested that the recombinant plasmid could be a DNA vaccine candidate.
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| 关 键 词： | 核酸疫苗 鼻腔免疫 基因疫苗 重组 表面蛋白 接种 表面蛋白 子孢子 |
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