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| 山羊生长激素基因部分序列的PCR-SSCP分析 | |
| 引用本文： | 李美玉,闵令江,潘庆杰,潘玉春,王天津.山羊生长激素基因部分序列的PCR-SSCP分析[J].中国畜牧杂志,2004,40(10):9-11. |
| 作者姓名： | 李美玉 闵令江 潘庆杰 潘玉春 王天津 |
| 作者单位： | 1. 莱阳农学院动物科学系,山东莱阳,265200 2. 上海交通大学农业与生物学院,上海,201101 3. 东营市绵野农业高科技有限责任公司,山东东营,257091 |
| 基金项目： | 山东省优秀中青年科学家科研奖励基金 |
| 摘 要： | 以鲁北白山羊、波尔山羊和波尔山羊与鲁北白山羊的杂交一代、回交一代共计 2 74个个体为研究材料 ,对山羊生长激素基因 5′调控区 - 4 0 6～ - 1 93片段进行PCR扩增 ,扩增产物经SSCP分析发现存在多态性。结果表明发现波尔山羊及杂交后代以AA型占多数 ,而鲁北白山羊则BB型个体较多 ,基因型频率在品种间存在显著差异 (P <0 .0 5 )。对该片段的两种纯合型克隆测序发现 :AA型在第 6 0位发生了C→T的突变 ,第 2 1 1位发生碱基C的丢失 |
| 关 键 词： | 畜牧、兽医科学基础学科 生长激素基因 PCR-SSCP 山羊 |
| 文章编号： | 0258-7033(2004)10-0009-03 |
| 修稿时间： | 2004年2月2日 |
PCR-SSCP Analysis of Growth Hormone Gene Partial Sequence in Goats |
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| LI Mei-yu,MIN Ling-jiang,PAN Qing-jie,PAN Yu-chun,WANG Tian-jin.PCR-SSCP Analysis of Growth Hormone Gene Partial Sequence in Goats[J].Chinese Journal of Animal Science,2004,40(10):9-11. | |
| Authors: | LI Mei-yu MIN Ling-jiang PAN Qing-jie PAN Yu-chun WANG Tian-jin |
| Institution: | LI Mei-yu~1,MIN Ling-jiang~1,PAN Qing-jie~1,PAN Yu-chun~2,WANG Tian-jin~3 |
| Abstract: | The polymorphism of -406～-193 DNA fragment of 5′ regulation region of growth hormone gene was detected by PCR-SSCP on 274 goats including Lubei White goat, Boer goat, hybrid generation 1 and backcross generation 1 of Boer goat and Lubei White goat. The result showed that most of the Boer goats and hybrid generation presented as AA genotype, while moer Lubei White goats presented as BB genotype. Statistical analysis indicated that the genotype frequence was different(P<0.05). The result of cloning and sequencing showed that AA genotype had two substitution mutations at 60 site (C→T) and base C lost at 211 site. |
| Keywords: | PCR-SSCP |
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