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| 高效液相色谱-串联质谱法测定水产品中玉米赤霉醇类激素药物残留量 | |
| 引用本文： | 钱卓真,刘智禹,邓武剑,魏博娟.高效液相色谱-串联质谱法测定水产品中玉米赤霉醇类激素药物残留量[J].南方水产,2011,7(1):62-68. |
| 作者姓名： | 钱卓真 刘智禹 邓武剑 魏博娟 |
| 作者单位： | 1. 福建省水产研究所,福建,厦门,361012;厦门大学材料学院,福建,厦门,361005 2. 福建省水产研究所,福建,厦门,361012 3. 集美大学生物工程学院,福建,厦门,361021 |
| 摘 要： | 文章建立用高效液相色谱-串联质谱法（HPLC—MS／MS）测定水产品中玉米赤霉醇类激素药物残留量的方法。用乙腈提取水产品中6种玉米赤霉醇类药物，正己烷脱脂，氨基固相萃取柱净化。采用电喷雾电离，负离子扫描，选择反应监测模式（SRM）监测，外标法定量。该法对6种玉米赤霉醇类药物标准曲线的线性回归系数均在0．99以上，线性范围0～25μg·kg^-1，方法定量限1．0μg·kg-1。6种玉米赤霉醇类激素药物回收率75．9％～103．8％，相对标准偏差3．90％～13．5％。该法简单、灵敏，结果可靠，可满足实验室批量样品分析的需求。 |
| 关 键 词： | 高效液相色谱-串联质谱法 玉米赤霉醇 水产品 残留 |
HPLC-MS/MS determination of zeranols residues in aquatic products |
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| QIAN Zhuozhen,LIU Zhiyu,DENG Wujian,WEI Bojuan.HPLC-MS/MS determination of zeranols residues in aquatic products[J].South China Fisheries Science,2011,7(1):62-68. | |
| Authors: | QIAN Zhuozhen LIU Zhiyu DENG Wujian WEI Bojuan |
| Institution: | 1. Fisheries Research Institute of Fujian, Xiamen 361012, China; 2. Biological Engineering College, Jimei University, Xiamen 361021 , China ; 3. College of Materials, Xiamen University, Xiarnen 361005, China) |
| Abstract: | We developed a method for determining zeranols residues such as α-zearalanol,β-zearalanol, α-zearalenol, β-zearalenol, zearalanone and zearalenone by high performance liquid chromatography-tandem mass spectrometry （ HPLC-MS/MS ）. The samples were extracted with acetonitrile, degreased with hexane and purified with amino solid-phase extraction column. In addition, with elec- trospray ionization in negative mode, we monitored the samples with selected reaction monitoring （SRM） and quantified them with ex- ternal standard method. For the 6 zeranols, it shows good linear regression cocffcient in the standard curve （ all 〉 0. 99 ） ; the linear range is 0 -25 μg-kg-1 ; the limit of detection is 1.0 μg·kg-1 ; the average recovery is 75.9% - 103.8% , and the relative standard deviation is 3.90 % - 13.5 %. The method, which is simple, sensitive and reliable, can be used to identify and quantify zeranols res- idues in aquatic products. |
| Keywords: | high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） zeranols aquatic products residues |
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