| 伯氏致病杆菌SN269发酵液中madumycin Ⅱ的分离与纯化 |
| |
| 引用本文： | 张春珍,石丹姝,张文波,席雪冬,于志国.伯氏致病杆菌SN269发酵液中madumycin Ⅱ的分离与纯化[J].农药学学报,2016,18(6):783-786. |
| |
| 作者姓名： | 张春珍 石丹姝 张文波 席雪冬 于志国 |
| |
| 作者单位： | 1.沈阳农业大学 植物保护学院, 沈阳 110866 |
| |
| 基金项目： | 沈阳农业大学引进人才启动基金. |
| |
| 摘 要： | 利用硅胶柱层析、凝胶柱层析技术从伯氏致病杆菌SN269发酵液中分离得到化合物D3。通过核磁共振波谱、质谱及旋光度等分析，并结合相关文献，将其鉴定为madumycinⅡ。采用菌丝生长速率法测定了madumycin Ⅱ对植物病原真菌的抑菌活性。结果表明:madumycin Ⅱ对辣椒疫霉病菌Phytophthoa capsici和番茄灰霉病菌Botrytis cinerea菌丝生长具有明显的抑制作用，其EC50值分别为35.32和35.40 mg/L。
|
| 关 键 词： | 致病杆菌 次生代谢产物 madumycin Ⅱ 分离纯化 抑菌作用 |
| 收稿时间： | 2016/5/23 0:00:00 |
| 修稿时间： | 2016/10/9 0:00:00 |
Isolation and purification of madumycin II from fermented broth of Xenorhabdus bovienii SN269 |
| |
| ZHANG Chunzhen,SHI Danshu,ZHANG Wenbo,XI Xuedong and YU Zhiguo.Isolation and purification of madumycin II from fermented broth of Xenorhabdus bovienii SN269[J].Chinese Journal of Pesticide Science,2016,18(6):783-786. |
| |
| Authors: | ZHANG Chunzhen SHI Danshu ZHANG Wenbo XI Xuedong and YU Zhiguo |
| |
| Institution: | 1.Colledge of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China2.Engineering & Technological Research Center of Biopesticide, Shenyang 110866, China |
| |
| Abstract: | Compound D3 was isolated from fermented broth ofXenorhabdus bovienii SN269 by silica gel column chromatography and Sephadex chromatography. The structure ofD3 was identified as madumycinⅡ, by comparing the1H NMR and13C NMR, ESI-MS and specific rotation data with the corresponding data reported in published literatures. The antifungal activity of madumycinⅡ against phytopathogenic fungi was determined by mycelium growth rate method. The results showed madumycinⅡ could effectively inhibit the mycelium growth ofPhytophthoa capsici andBotrytis cinerea, and the EC50 values were 35.32 mg/L and 35.40 mg/L, respectively. |
| |
| Keywords: | Xenorhabdus secondary metabolites madumycinⅡ isolation and purification antifungal activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《农药学学报》浏览原始摘要信息 |
| 点击此处可从《农药学学报》下载免费的PDF全文 |
