| 紫杉醇对G2/M期A549细胞4EBP1分子磷酸化的影响 |
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| 引用本文： | 李妍,于卉影,蔺迪,姜志明,马东初.紫杉醇对G2/M期A549细胞4EBP1分子磷酸化的影响[J].安徽农业科学,2010,38(1):1-3. |
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| 作者姓名： | 李妍 于卉影 蔺迪 姜志明 马东初 |
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| 作者单位： | 沈阳军区总医院医学实验科,辽宁沈阳,110016;吉林医药学院病原学教研室,吉林吉林,132013;沈阳军区总医院医学实验科,辽宁沈阳,110016 |
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| 摘 要： | 目的]研究不同浓度紫杉醇对A549细胞周期、凋亡和4EBP1分子磷酸化的影响。方法]采用不同浓度紫杉醇处理A549细胞，建立凋亡和G2／M期阻滞模型；分别用流式细胞术分析细胞凋亡和细胞周期；用免疫印迹方法分析4EBP1的表达与磷酸化。结果]G2／M期细胞百分率随紫杉醇浓度的增加而增加，但细胞凋亡率随紫杉醇浓度的增加而下降。随G2／M阻滞的增加，4EBP1分子3组丝／苏氨酸位点磷酸化均增强。结论j紫杉醇在G2／M期上调A549细胞内4EBP1分子多位点的磷酸化。
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| 关 键 词： | 紫杉醇 凋亡 真核翻译启始因子4结合蛋白 |
Effects of Paclitaxel on the Phosphorylation of 4EBP1 in A549 Cellls during G2/M Stage |
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| LI Yan et al.Effects of Paclitaxel on the Phosphorylation of 4EBP1 in A549 Cellls during G2/M Stage[J].Journal of Anhui Agricultural Sciences,2010,38(1):1-3. |
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| Authors: | LI Yan |
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| Institution: | Department of Experimental Medicine of Northern Hospital;Shengyang;Liaoning 110016 |
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| Abstract: | Objective]The paper aimed to study the effects of different concentrations of paclitaxel on the cell cycle and apoptois of A549 and phosphorylation of 4EBP1.Method]A cell apoptosis and mitosis(G2/M stage) retention model was established by treating A549 cell with different concentrations of paclitaxel.Cell apoptosis and cell cycle were determined by flow cytometry.The expression and phosphorylation of 4EBP1 were examined by western-blot.Result]The percentage of mitosis retention of G2/M stage cells raise... |
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| Keywords: | Paclitaxel Apoptosis 4EBP1 |
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