| 10种中药成分对CEF的增殖和抵抗NDV感染的影响 |
| |
| 引用本文： | 胡元亮,孔祥峰,李祥瑞,王德云,刘家国,张宝康,王效田.10种中药成分对CEF的增殖和抵抗NDV感染的影响[J].畜牧兽医学报,2004,35(3):301-305. |
| |
| 作者姓名： | 胡元亮 孔祥峰 李祥瑞 王德云 刘家国 张宝康 王效田 |
| |
| 作者单位： | 1. 南京农业大学动物医学院,南京,210095
2. 南京市浦口区畜牧兽医站,南京,210001 |
| |
| 基金项目： | 国家自然科学基金资助项目(30070566) |
| |
| 摘 要： | 分别将5种浓度的黄芪多糖(APS)、当归多糖(CAPS)、淫羊藿多糖(EPS)、板蓝根多糖(IRPS)、蜂胶多糖(PPS)、黄芪黄酮(AF)、淫羊藿黄酮(EF)、蜂胶黄酮(PF)、黄芪皂甙(AS)和人参皂甙(GS)10种中药成分加入到培养24h的鸡胚成纤维细胞(CEF)中，再培养12h后接种新城疫病毒(NDV)，于病毒接种后72h用中性红染料吸收法测定CEF活性以评价各中药成分对细胞增殖及其抵抗病毒感染的影响。结果表明，10种中药成分均能不同程度地促进细胞增殖和抵抗病毒感染，前者以AF、APS、IRPS、PF、和GS的作用较强，后者以GS和IRPS的作用较强，部分中药成分在两方面作用中各有所长，且有一定的量效关系。
|
| 关 键 词： | 中药成分 鸡胚成纤维细胞 细胞增殖 抗病毒作用 免疫增强剂 |
| 文章编号： | 0366-6964(2004)03-0301-05 |
Effects of Ten Chinese Herbal Medicinal Ingredients on Proliferation and Resisting NDV Infection of CEF |
| |
| HU Yuan-liang,KONG Xiang-feng,LI Xiang-rui,WANG De-yun,LIU Jia-guo,ZHANG Bao-kang,WANG Xiao-tian.Effects of Ten Chinese Herbal Medicinal Ingredients on Proliferation and Resisting NDV Infection of CEF[J].Acta Veterinaria et Zootechnica Sinica,2004,35(3):301-305. |
| |
| Authors: | HU Yuan-liang KONG Xiang-feng LI Xiang-rui WANG De-yun LIU Jia-guo ZHANG Bao-kang WANG Xiao-tian |
| |
| Institution: | HU Yuan-liang~1,KONG Xiang-feng~1,LI Xiang-rui~1,WANG De-yun~1,LIU Jia-guo~1,ZHANG Bao-kang~1,WANG Xiao-tian~2 |
| |
| Abstract: | Ten Chinese herbal medicinal ingredients, astragalus polysaccharide(APS), Chinese angelica polysaccharide(CAPS), epimedium polysaccharide(EPS), isatis root polysaccharide(IRPS), propolis polysaccharide (PPS), astragalus flavone (AF), epimedium flavone (EF), propolis flavone (PF), astragalosides (AS) and ginsenosides (GS), were added into chick embryo fibroblast (CEF), which were cultured for 24 hours, at five concentration respectively. After 12 hours, the cell was inoculated with Newcastle disease virus (NDV). At 72 hours after inoculation, the cellular activity was tested by Neutral Red Assay to evaluate the effects of the ten ingredients on proliferation and antiviral infection of the cell. The results showed that all of the ten could promote the cell proliferation and resisting the virus infection in different degrees. The better was AF,APS,IRPS,PF and GS in proliferation-promoting and GS and IRPS in antiviral infection. Some ingredients had their stronger one in two actions. There were certain dose-effect relationships in these actions. |
| |
| Keywords: | Chinese herbal medicinal ingredient Cellular proliferation Antiviral action |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
