Dietary branched‐chain amino acid supplementation affects growth and hepatic insulin‐like growth factor gene expression in yellowtail,Seriola quinqueradiata |
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| Authors: | M Kawanago S Takemura R Ishizuka I Shioya |
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| Institution: | 1. Aquaculture Fundamental Research Group, Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Hachioji, Tokyo, Japan;2. Research Support & Administration Section, Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Hachioji, Tokyo, Japan |
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| Abstract: | We investigated the effect of dietary supplementation with a branched‐chain amino acid (BCAA) mixture (valine, leucine and isoleucine) on juvenile yellowtail (Seriola quinqueradiata) growth and hepatic insulin‐like growth factor (IGF) gene expression. Total dietary BCAA content was 8.1, 8.5, 9.0 and 9.8 g kg?1 for the control, 0.4%, 1.0% and 2.0% BCAA experimental groups, respectively. Body weight was measured on day 77, after which fish were fasted for 3 days and refed; the livers were then removed 3 or 9 h later for analysis of hepatic gene expression. Body weight significantly increased in the BCAA‐supplemented groups. Hepatic expression of IGF1 and IGF2 significantly increased in the 2% BCAA group at 3 and 9 h after refeeding. In the 1% BCAA group, hepatic IGF1 expression tended to be higher at 9 h than in the control group after refeeding. Also, hepatic IGF2 significantly increased at only 9 h after refeeding in the 1% BCAA group. In conclusion, dietary supplementation with crystalline BCAAs increased growth and hepatic expression of IGF1 and IGF2. These results suggest that dietary crystalline BCAA supplementation would be a valuable addition to yellowtail aquaculture practices. |
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| Keywords: | branched‐chain amino acid feed efficiency ratio gene expression insulin‐like growth factor
Seriola quinqueradiata
yellowtail |
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