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| 李矮缩病毒RT-PCR方法建立及检测应用 | |
| 引用本文： | 侯义龙,杨俊玲,李春敏.李矮缩病毒RT-PCR方法建立及检测应用[J].中国农业科学,2005,38(2):425-427. |
| 作者姓名： | 侯义龙 杨俊玲 李春敏 |
| 作者单位： | 1. 大连大学生物工程学院,大连,116622 2. 中国农业科学院果树研究所,兴城,125100 3. 河北省农林科学院昌黎果树研究所,昌黎,066600 |
| 基金项目： | 辽宁省博士启动基金资助项目(20021052)，辽宁省教育厅高等学校科研项目(2023901007) |
| 摘 要： | 以感病和健康指示植物GF305的总RNA为模板，进行cDNA的合成和PCR 扩增，结果从感病材料中扩增出与预期的172 bp大小一致的目的片段，而健康的材料无此扩增产物。对此PCR 扩增产物克隆测序，进一步佐证了RT-PCR检测结果。经过多次试验验证该检测体系，都可得到很好的重演性，从而建立了李矮缩病毒快速、灵敏、准确的RT-PCR检测技术，并进行了实际应用。 |
| 关 键 词： | 李矮缩病毒 逆转录－聚合酶链式反应 克隆与测序 |
| 收稿时间： | 2004-2-3 |
Establishment and Application of Detection of Prune Dwarf Virus (PDV) by RT-PCR |
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| HOU Yi-long,YANG Jun-ling,LI Chun-min.Establishment and Application of Detection of Prune Dwarf Virus (PDV) by RT-PCR[J].Scientia Agricultura Sinica,2005,38(2):425-427. | |
| Authors: | HOU Yi-long YANG Jun-ling LI Chun-min |
| Institution: | HOU Yi-long1,YANG Jun-ling2,LI Chun-min3 |
| Abstract: | A pair of primers was synthesized based on the nucleotide sequence of the coat protein gene of prune dwarf virus (PDV).The expected size 172bp was amplified by RT-PCR from the infected samples, while no amplified products from the healthy samples.The amplified products were cloned and sequenced. The result showed that the detection system was stable.Thus a rapid, sensitive and accurate method for PDV identification and detection by RT-PCR has been built up and were used to detect whether some fruit trees in orchards were infected by PDV or not. |
| Keywords: | Prune dwarf virus RT-PCR Cloning and sequencing |
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