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番鸭小鹅瘟病胶乳凝集试验与病毒分离的比较研究
引用本文:朱小丽,林锋强,陈少莺,程晓霞,王燕玲,陈仕龙,王劭,李兆龙.番鸭小鹅瘟病胶乳凝集试验与病毒分离的比较研究[J].畜牧兽医杂志,2012,31(2):9-10,12.
作者姓名:朱小丽  林锋强  陈少莺  程晓霞  王燕玲  陈仕龙  王劭  李兆龙
作者单位:1. 福建省农业科学院畜牧兽医研究所,福州350013/福建省畜禽疫病防治工程技术研究中心
2. 福建省农业科学院畜牧兽医研究所,福州,350013
基金项目:福建省农业科学院创新团队(STIF-Y02); 福建省公益类项目(2009R10025-6); 国家公益类(农业)科研专项经费项目(201003012)
摘    要:应用GPV胶乳凝集方法(LPA)和病毒分离(VI)检测人工感染病例9份、健康对照6份,临床疑似送检病鸭28份。其中人工感染样品检测LPA和VI符合率为93.3%(14/15);临床疑似样品检测LPA和VI符合率为89.3%(25/28);两者总符合率达90.7%(39/43)。LPA方法具有简便(肉眼可判定,不需仪器)、快速(检测抗原20min内)、特异、准确等优点,比VI更适合临床使用。

关 键 词:番鸭小鹅瘟病  胶乳诊断  病毒分离  符合率

Comparison Study on Latex Agglutination Assay and Virus Isolation for Detecting Muscovy Duck Gosling Plague
ZHU Xiao-li,LIN Feng-qiang,CHEN Shao-ying,CHENG Xiao-xia,WANG Yan-ling,CHEN Shi-long,WANG Shao,LI Zhao-long.Comparison Study on Latex Agglutination Assay and Virus Isolation for Detecting Muscovy Duck Gosling Plague[J].Journal of Animal Science and Veterinary Medicine,2012,31(2):9-10,12.
Authors:ZHU Xiao-li  LIN Feng-qiang  CHEN Shao-ying  CHENG Xiao-xia  WANG Yan-ling  CHEN Shi-long  WANG Shao  LI Zhao-long
Institution:1,2,(1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences,Fuzhou Fujian 350013; 2.Fujian Animal Diseases Control Technology Development Center)
Abstract:Nine duck cases experimentally infected with GPV,6 healthy duck cases and 18 clinically suspicious samples were detected by GPV latex agglutination assay(LPA) and virus isolation(VI).The coincidence rate of LPA and VI was 93.3%(14/15)for 15 experimental samples.That was 89.3%(25/28)for clinical samples.Total coincidence rate of was LPA and VI was 90.7%(39/43).LPA was a practical method for clinical diagnosis of GPV disease based on its simplicity(judged with eye only),rapidity(20min) and specificity nature.
Keywords:Latex agglutination assay  virus isolation  comparsion
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