| 小尾寒羊MTNR1A基因外显子2的克隆与序列分析 |
| |
| 引用本文： | 程笃学,储明星,刘文忠,方丽,叶素成.小尾寒羊MTNR1A基因外显子2的克隆与序列分析[J].农业生物技术学报,2006,14(2):166-169. |
| |
| 作者姓名： | 程笃学 储明星 刘文忠 方丽 叶素成 |
| |
| 作者单位： | 1. 山西农业大学动物科技学院,太谷,030801
2. 中国农业科学院畜牧研究所,北京,100094 |
| |
| 摘 要： | 对常年发情的绵羊品种小尾寒羊和季节性发情的绵羊品种多赛特羊共20只母羊的褪黑激素受体1A(melatonin receptor 1A，MTNR1A)基因外显子2的824 bp扩增产物进行了克隆测序及序列比较分析。结果表明, 小尾寒羊MTNR1A基因外显子2的核苷酸序列与已发表的绵羊序列(GenBank登录号U14109)完全相同。小尾寒羊与多赛特羊MTNR1A基因外显子2的差异由5个变化的核苷酸(分别为C329T、G355T、C566T、C580A和A675G)组成，核苷酸同源性为99.4%。小尾寒羊与山羊、母牛、猪、人、小鼠、挪威大鼠、西伯利亚仓鼠和鸡之间MTNR1A基因外显子2的核苷酸序列同源性为73.2%～98.5%，氨基酸序列同源性为78.5%～98.5%。
|
| 关 键 词： | 绵羊 繁殖季节性 褪黑激素受体1A基因 克隆 序列分析 |
| 文章编号： | 1006-1304（2006）02-0166-04 |
| 收稿时间： | 2005-1-22 |
| 修稿时间： | 2005-02-02 |
Cloning and Sequence Analysis of Exon 2 of MTNR1A Gene in Small Tail Han Sheep |
| |
| CHENG Du-xue,CHU Ming-xing,LIU Wen-zhong,FANG Li,YE Su-cheng.Cloning and Sequence Analysis of Exon 2 of MTNR1A Gene in Small Tail Han Sheep[J].Journal of Agricultural Biotechnology,2006,14(2):166-169. |
| |
| Authors: | CHENG Du-xue CHU Ming-xing LIU Wen-zhong FANG Li YE Su-cheng |
| |
| Institution: | 1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801,China; 2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China |
| |
| Abstract: | The 824 bp fragment of exon 2 of melatonin receptor 1A(MTNR1A ) gene was amplified successfully by PCR in both 10 ewes of nonseasonal estrous breed (Small Tail Han sheep) and 10 ewes of seasonal estrous breed (Dorset sheep), and then cloned into pGEM-T Easy vector. The positive clones were further identified by PCR and sequenced. The nucleotide sequence of exon 2 of MTNR1A gene in Small Tail Han sheep was the same as that published in GenBank (U14109). The differences in exon 2 of MTNR1A gene between Small Tail Han sheep and Dorset sheep consisted of five nucleotide changes (C329T, G355T, C566T, C580A and A675G), and homology of nucleotide sequence was 99.4%. Homologies of nucleotide sequence and amino acid sequence of exon 2 of MTNR1A gene between Small Tail Han sheep and goat, cow, pig, human, house mouse, Norway rat, Siberian hamster and chicken were 73.2% to 98.5% and 78.5% to 98.5%, respectively. |
| |
| Keywords: | sheep reproductive seasonality melatonin receptor 1A gene cloning sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《农业生物技术学报》浏览原始摘要信息 |
|
