| 梨自交不亲和新基因S35-RNase的克隆与表达分析 |
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| 引用本文： | 杨谷良,谭晓风.梨自交不亲和新基因S35-RNase的克隆与表达分析[J].园艺学报,2007,34(3):751-754. |
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| 作者姓名： | 杨谷良 谭晓风 |
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| 作者单位： | (中南林业科技大学经济林育种与栽培国家林业局重点实验室, 长沙410004; 黄冈师范学院生命科学与工程学院,湖北黄冈438000) |
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| 基金项目： | 国家林业局重点科学技术开发与储备专项基金;湖北省自然科学基金;湖南省科技三项费资助项目 |
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| 摘 要： | 通过RACE技术, 从早酥梨花柱中分离到一个长度为681 bp的自交不亲和基因, DNA序列分析表明, 其开放读码框由227个氨基酸组成, 具有S2RN ase基因特有的保守组氨酸和半胱氨酸残基、高变区和保守区等特征序列, 与己登录的S4-RNase基因DNA序列同源性最高, 达94% , 登录为一个新基因:S35-RNase, GenBank接收号为DQ224344。通过Northern杂交, 发现该基因只在花柱中特异性表达, 并且在大铃铛期的表达量比花前和花后3 d的表达量都高。
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| 关 键 词： | 梨 RACE 自交不亲和性 S35-RNase Northern杂交 |
| 文章编号： | 0513-353X（2007）03-0751-04 |
| 修稿时间： | 2006-10-082007-04-17 |
Cloneand Expression Analysis of a Novel S-allele: S35-RNase in Pyrus bretschneideri |
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| YANG Gu-liang,TAN Xiao-feng.Cloneand Expression Analysis of a Novel S-allele: S35-RNase in Pyrus bretschneideri[J].Acta Horticulturae Sinica,2007,34(3):751-754. |
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| Authors: | YANG Gu-liang TAN Xiao-feng |
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| Institution: | (The Key Laboratory of Non-wood Forest Products of the Ministry of Forestry, Central South University of Forestry and Technology, Changsha 410004, China; Biology Department, Huanggang Normal University, Huanggang, Hubei 438000,China) |
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| Abstract: | A new self-incompatibility gene,which contains an open reading frame of 681 nucleotides encoding a 227 amino acid protein,was isolated from Pyrus bretschneideri cultivars of Zaosu by rapid amplification of cDNA ends(RACE).DNA sequence analysis revealed that the isolated gene contains the specific sequences of S-RNase,such as conserved histidines and cysteines,hypervariable region and conserved regions.It displayed the highest homology of 94% with the embodied S4-RNase(AB014072).The new S-allele was named S35-RNase and its accession number was DQ224344 in GenBank.The northern blot analysis indicated that this gene only expressed in pistil,with a higher expression level at the balloon stage than at the stage of 3 days before or after flowering. |
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| Keywords: | RACE S35-RNase |
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