Establishment of cell suspension line of Populus tomentosa Carr |
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| Authors: | YAO Na ZHANG Zhi-yi AN Xin-min YANG Kai |
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| Institution: | 1. Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Minl.Coy of Education, Beijing Forestry University, Beijing 100083, P. R. China
2. Department of Biotechnology, Beijing Agricultural College, Beijing 102206, P. R. China |
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| Abstract: | Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subeultured into a MS liquid medium supplemented with 1.5 mg·L-1 2,4-D. The best subculture medium was MS 0.8 mg·L-1 2,4-D 30 g·L-1 sucrose with a subculture cycle of seven days. |
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| Keywords: | Populus tomentosa callus cell suspension line |
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