| 多环境条件下大豆异黄酮主要组分的QTL定位 |
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| 引用本文： | 张晶莹,葛一楠,孙君明,韩粉霞,于福宽,闫淑荣,杨华.多环境条件下大豆异黄酮主要组分的QTL定位[J].中国农业科学,2012,45(19):3909-3920. |
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| 作者姓名： | 张晶莹 葛一楠 孙君明 韩粉霞 于福宽 闫淑荣 杨华 |
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| 作者单位： | 1.中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室 北京 100081 |
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| 基金项目： | 转基因重大专项(2011ZX08004-003);国家自然科学基金(31171576);国家“十二五”科技支撑计划(2011BAD35B06-3) |
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| 摘 要： | 【目的】利用不同定位方法，对不同环境条件下大豆异黄酮主要组分进行QTL定位研究，为大豆异黄酮分子标记辅助育种提供理论依据。【方法】以异黄酮含量有显著差异的鲁黑豆2号（3 697.24 μg•g-1）和南汇早黑豆（1 816.67 μg•g-1）为亲本构建的F5∶7-8重组自交系为材料，分析RIL群体的SSR标记多态性，结合HPLC法鉴定异黄酮主要组分含量。【结果】绘制了一张包含161个多态性SSR标记，全长3 546.54 cM的大豆遗传连锁图谱。利用ICIMapping 3.2软件的ICIM、IM和SMA 3种定位方法，共定位到4种环境下与异黄酮主要组分相关的14个QTL。【结论】3个标记区间在多个环境和多种定位方法下均被检测到，分别是Sat_003—Satt306、Satt070—Satt122和Satt571—Satt270。
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| 关 键 词： | 大豆 异黄酮 SSR QTL |
| 收稿时间： | 2012-06-11 |
Identification of QTLs for Major Isoflavone Components Among Multiple Environments in Soybean Seeds |
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| ZHANG Jing-ying,GE Yi-nan,SUN Jun-ming,HAN Fen-xia,YU Fu-kuan,YAN Shu-rong,YANG Hua.Identification of QTLs for Major Isoflavone Components Among Multiple Environments in Soybean Seeds[J].Scientia Agricultura Sinica,2012,45(19):3909-3920. |
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| Authors: | ZHANG Jing-ying GE Yi-nan SUN Jun-ming HAN Fen-xia YU Fu-kuan YAN Shu-rong YANG Hua |
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| Institution: | (Institute of Crop Science,Chinese Academy of Agricultural Sciences/Ministry of Agriculture(MOA) Key Laboratory of Soybean Biology,Beijing 100081) |
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| Abstract: | 【Objective】The QTLs for major isoflavone components were identified among multiple environments by different QTL mapping methods in soybean seeds, in order to provide a theoretical basis for soybean isoflavone marker-assisted selection. 【Method】 In this study, a recombinant inbreed line population (RIL, F5∶7-8) was developed by using the cross between the isoflavone contrasting cv. LHD2 with high-isoflavone concentration (3 697.24 μg•g-1) and cv. NHZ with low-isoflavone concentration (1 816.67 μg•g-1). The polymorphism of SSR markers among the RIL population was analyzed and the major isoflavone components were determined by HPLC. 【Result】The results showed that a soybean linkage map with a total distance of 3 546.54 cM was constructed using 161 polymorphism SSR molecular markers in the RIL population. A total of 14 QTLs associated with the major isoflavone components were found by ICIM, IM, and SMA methods in the ICIMapping 3.2 software under the four environments. 【Conclusion】The three QTLs flanked by the marker interval Sat_003-Satt306, Satt070-Satt122 and Satt571-Satt270 were detected among multiple environments in more than two methods. |
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| Keywords: | soybean (Glycine max L Merrill isoflavone component SSR QTL |
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