A genome‐wide SNP scan in a porcine Large White × Minzhu intercross population reveals a locus influencing muscle mass on chromosome 2 |
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| Authors: | Xin Liu Li Gang Wang Wei Zhen Luo Yong Li Jing Liang Hua Yan Ke Bin Zhao Li Xian Wang Long Chao Zhang |
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| Institution: | 1. Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, , Beijing, China;2. Animal Husbandry Research Institute, Beijing Sanyuan Breeding Technology Co., Ltd, , Beijing, China;3. Chuying Agro‐pastoral Group Co., Ltd, , Xinzheng, China |
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| Abstract: | A high‐density single nucleotide polymorphism (SNP) array containing 62 163 markers was employed for a genome‐wide association study (GWAS) to identify variants associated with lean meat in ham (LMH, %) and lean meat percentage (LMP, %) within a porcine Large White × Minzhu intercross population. For each individual, LMH and LMP were measured after slaughter at the age of 240 ± 7 days. A total of 557 F2 animals were genotyped. The GWAS revealed that 21 SNPs showed significant genome‐wide or chromosome‐wide associations with LMH and LMP by the Genome‐wide Rapid Association using Mixed Model and Regression‐Genomic Control approach. Nineteen significant genome‐wide SNPs were mapped to the distal end of Sus Scrofa Chromosome (SSC) 2, where a major known gene responsible for muscle mass, IGF2 is located. A conditioned analysis, in which the genotype of the strongest associated SNP is included as a fixed effect in the model, showed that those significant SNPs on SSC2 were derived from a single quantitative trait locus. The two chromosome‐wide association SNPs on SSC1 disappeared after conditioned analysis suggested the association signal is a false association derived from using a F2 population. The present result is expected to lead to novel insights into muscle mass in different pig breeds and lays a preliminary foundation for follow‐up studies for identification of causal mutations for subsequent application in marker‐assisted selection programs for improving muscle mass in pigs. |
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| Keywords: | genome‐wide association study lean meat in ham lean meat percentage pig |
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