Cryopreservation of Mekong catfish,Pangasius bocourti Sauvage, 1880 spermatozoa |
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| Authors: | Supannee Kainin Samorn Ponchunchoovong Unnop Imsilp Sombut Singsee |
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| Institution: | 1. Institute of Agricultural Technology, Suranaree University of Technology, , Nakhon Ratchasima, 30000 Thailand;2. Department of Fisheries, Nong Khai Inland Fisheries Research and Development Center, , Si Chiang Mai, Nong Khai, 43130 Thailand;3. Department of Fisheries, Nakhonphanom Inland Fisheries Research and Development Center, , Amphoe Muang, Nakhon Phanom, 48000 Thailand |
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| Abstract: | The effects of three extenders (Ginzburg fish ringer, Calcium‐free Hank's balanced salt solution, C‐F HBSS and sodium chloride, 0.9% NaCl) and four cryoprotectants (dimethyl sulphoxide, DMSO; dimethyl acetamide, DMA; methanol, MeOH and glycerol) in different concentrations (5%, 10% and 15%) on the motility, viability and fertilization rates of Mekong catfish (Pagasius bocourti) sperm were investigated. Sperm samples were transferred into 250‐μL French straws and sealed with a heated haemostat. The straws were then placed in a cryochamber. A computer‐controlled rate freezer (CL 3300) and programmable Cryogenesis, version 4 were used to regulate the freezing rate. The sperm samples were frozen at a rate of 10°C min?1 from 4 to ?80°C and then evaluated after 72 h. Of the three extenders used with each cryoprotectant, C‐F HBSS had the highest fertilization rate of 75% (93% of control). This was not significantly different from the control treatment (fresh sperm) when tested with DMSO as the cryoprotectant. The lowest fertilization rate of 27% (38% of control) was resulting from the combination of 15% glycerol and C‐F HBSS. This study found that fertilization, motility and viability rates in all of the experiments had a positive significant correlation (P < 0.001). |
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| Keywords: | Cryopreservation extender cryoprotectant sperm Mekong catfish
Pangasius bocourti
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