A proteomic analysis of Nipponia nippon (ID#162) |
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| Authors: | Mitsuru Oyanagi Kentaro Kaneko Yoshinori Kaneko Maiko Sasaki Chizuko Nishida Yoichi Matsuda Toshiaki Mitsui |
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| Institution: | 1. Genome Research Center, Faculty of Agriculture, Niigata University, , Niigata, Japan;2. Graduate School of Science and Technology, Niigata University, , Niigata, Japan;3. Center for Toki and Ecological Restoration, Niigata University, , Niigata, Japan;4. Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, , Niigata, Japan;5. Sado Japanese Crested Ibis Conservation Center, , Sado, Japan;6. Department of Natural History Sciences, Faculty of Science, Hokkaido University, , Sapporo, Japan;7. Avian Bioscience Research Center (ABRC), Graduate School of Bioagricultural Sciences, Nagoya University, , Nagoya, Japan |
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| Abstract: | We investigated the proteome of a female Crested Ibis (Nipponia nippon, ID#162) that died on March 10, 2010 at the Sado Japanese Crested Ibis Conservation Center. Protein preparations from the brain, trachea, liver, heart, lung, proventriculus, muscular stomach, small intestine, duodenum, ovary and neck muscle were subjected to in‐solution shotgun mass spectrometry (MS)/MS analyses using an LTQ Orbitrap XL mass spectrometer. A search of the National Center for Biotechnology Information Gallus gallus databases revealed 4253 GI (GenInfo Identifier) numbers with the sum of the same 11 tissues examined in the Crested Ibis. To interpret the obtained proteomics data, it was verified in detail with the data obtained from the brain of the Crested Ibis. It has been reported that drebrin A is specifically expressed in adult chicken brain. In the shotgun proteomic analyses of the Crested Ibis, we identified drebrin A as a brain‐specific protein. Furthermore, Western blotting analysis of the protein preparations from 10 tissues of the Crested Ibis and 150‐day‐old hens using anti‐drebrin antibodies showed intensive expression of approximately 110 kDa polypeptides of drebrin in both brains. We believe firmly that the present data will contribute to initial and fundamental steps toward understanding the Crested Ibis proteome. |
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| Keywords: | Crested Ibis drebrin A Nipponia nippon shotgun proteomic and LC‐MS/MS |
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