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广佳安对油菜芽苗根系形态结构及生理指标的影响
引用本文:董改改,慕小倩,汪梦竹,胡胜武.广佳安对油菜芽苗根系形态结构及生理指标的影响[J].中国油料作物学报,2015,37(2):206-213.
作者姓名:董改改  慕小倩  汪梦竹  胡胜武
作者单位:1.西北农林科技大学生命科学学院,陕西 杨凌,712100;2.西北农林科技大学农学院,陕西 杨凌,712100
基金项目:国家转基因专项(2008ZX08004-004);国家自然科学基金(31171574,31371646);大豆产业技术体系(CARS-004)
摘    要:为研究除草剂广佳安对油菜根系的影响,以甘蓝型油菜陕油16和白菜型油菜白杂一号为材料,用室内水培法研究了不同浓度广佳安对2个油菜品种芽苗根系生长发育的影响。结果表明,广佳安明显抑制油菜根系生长,随着处理浓度的增加抑制作用增大;广佳安使油菜芽苗主根长度、根干重及根冠比都下降,根毛和侧根长度缩短,根毛密度减小,且扭曲、变形,交错缠绕,分布不均匀。芽苗所测根系的MDA含量、过氧化物酶(POD)及过氧化氢酶(CAT)的活性都随广佳安处理浓度的增加而增大,超氧化物歧化酶(SOD)表现为先增高后降低的趋势。广佳安胁迫使根尖膨胀变粗,根冠由尖锥形变为近圆弧形,在较高浓度下,表皮细胞排列不整齐,甚至部分缺失;皮层薄壁组织细胞层数减少、细胞液泡化程度增高,细胞边缘皱褶溃烂。2个油菜品种相比,白杂一号对广佳安的耐药性较陕油16强。

关 键 词:油菜品种  除草剂广佳安  根系形态  生理响应  耐药性

Attack effects on morphological structure and physiological change of rape seedling root
DONG Gai-gai,MU Xiao-qian,WANG Meng-zhu,HU Sheng-wu.Attack effects on morphological structure and physiological change of rape seedling root[J].Chinese Journal of Oil Crop Sciences,2015,37(2):206-213.
Authors:DONG Gai-gai  MU Xiao-qian  WANG Meng-zhu  HU Sheng-wu
Institution:DONG Gai-gai;MU Xiao-qian;WANG Meng-zhu;HU Sheng-wu;College of Life Science,Northwest A&F University;College of Agronomy,Northwest A&F University;
Abstract:Responses of seedling root to herbicide Attack were investigated under indoor hydroponics culture using 2 cultivars (Brassica napus cv Shaanyou 16 and B. rapa cv Baizayihao. Results showed that inhibitive effects of Attack on root were enhanced with increased herbicide concentrations. Main root length, root dry weight and root-shoot ratio were depressed. Root hair and lateral root lengths were shortened. Root hair density was reduced, root hair shape was distorted and their distributions were uneven. Moreover, MDA, POD and CAT activities of seedling roots were ascended with the increased herbicide concentrations. SOD activity increased first and dropped later. Attack swelled and thickened root tips. Root cap shape changed from taper to circular arc. High concentration of Attack led to disorganized array of epidermal cells and part of the cell missed. Cortical cell layers were lessened, cell vacuolation degree increased and cell edge was damaged. Results also showed that Baizayihao held stronger tolerance to Attack stress than that of Shaanyou 16.
Keywords:Rape  Attack stress  Root morphology  Physiological response  Drug resistance
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