| 共表达K88/987p菌毛产肠毒素性大肠杆菌的分离鉴定及其卵黄抗体的制备 |
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| 引用本文： | 彭姣,李职,祁振强,卢超.共表达K88/987p菌毛产肠毒素性大肠杆菌的分离鉴定及其卵黄抗体的制备[J].中国畜牧兽医,2015,42(2):303-308. |
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| 作者姓名： | 彭姣 李职 祁振强 卢超 |
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| 作者单位： | 1. 深圳市宝舜泰生物医药股份有限公司, 深圳 518057;2. 深圳比利美英伟营养饲料有限公司, 深圳 518103 |
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| 基金项目： | 深圳市科创委技术创新计划专项基金资助项目(CXZZ20130320155104079) |
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| 摘 要： | 为制备针对一些大型养猪场产肠毒素性大肠杆菌(enterotrxigenic Escherichia coli,ETEC)分离株的特异性卵黄抗体(egg yolk immunoglobulin,IgY),试验对从这些养殖场分离的ETEC分离株菌毛基因类型进行了PCR鉴定,纯化该分离株的菌毛蛋白免疫蛋鸡制备IgY,对该IgY的效价、特异性和体外抑菌效果进行了检测。结果表明,该分离株具有K88和987p 两种菌毛基因,纯化后的分离株菌毛具有较强的免疫原性,经3次免疫后产生的IgY对K88和987p全菌和菌毛的效价可达到1:64 000,分离株菌毛IgY能特异地与K88和987p反应,与K99、F41无交叉反应,5 mg/mL分离株菌毛IgY在体外具有很好的抑菌效果。
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| 关 键 词： | K88 987p 共表达 菌毛蛋白 卵黄抗体 体外抑菌 |
| 收稿时间： | 2014-08-06 |
Isolation of the ETEC Strain Co-expressed K88/987p Fimbriae and Preparation of its Yolk Antibodies |
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| PENG Jiao,LI Zhi,QI Zhen-qiang,LU Chao.Isolation of the ETEC Strain Co-expressed K88/987p Fimbriae and Preparation of its Yolk Antibodies[J].China Animal Husbandry & Veterinary Medicine,2015,42(2):303-308. |
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| Authors: | PENG Jiao LI Zhi QI Zhen-qiang LU Chao |
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| Institution: | 1. Shenzhen Boostie Biological Pharmaceutical Co., Ltd., Shenzhen 518057, China;2. Shenzhen Premixinve Nutrition Co., Ltd., Shenzhen 518103, China |
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| Abstract: | For preparation of specific IgY against the ETEC strains from the pig farms,types of fimbriae gene of an ETEC strain isolated from these pig farms were identified by PCR.The pilin was purified and used to immunize hens to obtain the IgY.The titer,specificity and in vitro inhibitory effect of IgY were detected.The results showed that this ETEC strain had two types of fimbriae gene of K88 and 987p,and the immunogenic of the strain fimbriae was high.The titers of the isolates fimbriae IgY against the K88 and 987p fimbriae were both 1:64 000.The IgY could bind with K88 and 987p but could not either with K99 or F41. 5 mg/mL fimbriae IgY could completely inhibit the growth of K88 and 987p in vitro. |
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| Keywords: | K88 987p co-expression pilin IgY in vitro inhibition |
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