| 基因Ⅳ型戊型肝炎病毒开放阅读框3表达的蛋白(pORF3)对L02细胞基质金属蛋白酶(MMP)表达的影响 |
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| 引用本文： | 郭莳雨,史巧芸,贾晓晓,朱华培,赵天靖,庞峰,徐开莲,梁源祥,崔克,杜丽,吴科榜,王凤阳.基因Ⅳ型戊型肝炎病毒开放阅读框3表达的蛋白(pORF3)对L02细胞基质金属蛋白酶(MMP)表达的影响[J].中国畜牧兽医,2015,42(4):804-809. |
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| 作者姓名： | 郭莳雨 史巧芸 贾晓晓 朱华培 赵天靖 庞峰 徐开莲 梁源祥 崔克 杜丽 吴科榜 王凤阳 |
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| 作者单位： | 1. 海南大学农学院, 海南省热带动物繁育与疫病研究重点实验室, 海口市动物基因工程重点实验室, 海口 570228;2. 海南省动物疫病预防控制中心, 海口 570228 |
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| 基金项目： | 国家自然科学基金(31360618);海南省重点科技计划项目(ZDXM2014026);海南省社发专项(2012SF016);海口市重点科技项目(2010-048) |
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| 摘 要： | 为了探讨基因Ⅳ型戊型肝炎病毒开放阅读框3(ORF3)表达的蛋白(pORF3)影响靶细胞基质金属蛋白酶(MMP)表达的分子机制,试验运用蛋白芯片技术对比分析了稳定表达pEGFP-ORF3和pEGFP的L02细胞中基质金属蛋白酶表达谱,筛选出表达水平上调/下调的基质金属蛋白酶。结果表明,在稳定表达pEGFP-ORF3的L02细胞中,TIMP-1、TIMP-2和TIMP-4表达水平均降低。本试验结果为戊型肝炎病毒pORF3对L02细胞中基质金属蛋白酶相关蛋白表达影响的分子机制研究提供了理论依据。
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| 关 键 词： | 蛋白芯片 戊型肝炎 L02细胞 基质金属蛋白酶 表达 |
| 收稿时间： | 2014-09-09 |
Effects of Open Reading Frame 3 (ORF3) Expressed Protein of Genotype Ⅳ Hepatitis E Virus on the Expression of Matrix Metalloproteinase in L02 Cells |
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| GUO Shi-yu,SHI Qiao-yun,JIA Xiao-xiao,ZHU Hua-pei,ZHAO Tian-jing,PANG Feng,XU Kai-lian,LIANG Yuan-xiang,CUI Ke,DU Li,WU Ke-bang,WANG Feng-yang.Effects of Open Reading Frame 3 (ORF3) Expressed Protein of Genotype Ⅳ Hepatitis E Virus on the Expression of Matrix Metalloproteinase in L02 Cells[J].China Animal Husbandry & Veterinary Medicine,2015,42(4):804-809. |
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| Authors: | GUO Shi-yu SHI Qiao-yun JIA Xiao-xiao ZHU Hua-pei ZHAO Tian-jing PANG Feng XU Kai-lian LIANG Yuan-xiang CUI Ke DU Li WU Ke-bang WANG Feng-yang |
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| Institution: | 1. Animal Genetic Engineering Key Laboratory of Haikou City, Hainan Key Laboratory of Tropical Animal Reproduction, Breeding and Epidemic Disease Research, College of Agriculture, Hainan University, Haikou 570228, China;2. Hainan Animal Disease Prevention and Control Center, Haikou 570228, China |
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| Abstract: | To discuss the mechanism how open reading frame 3 (ORF3) protein of genotype Ⅳ hepatitis E virus (HEV) affected the expression of matrix metalloproteinase (MMP) in target cells, Human Matrix Metalloproteinase Antibody Array was used to compare and analyze the expression levels of matrix metalloproteinase in stable cell lines that expressed or did not express HEV ORF3, screening the matrix metalloproteinases which were down-regulated or up-regulated. The results indicated that the expressions of TIMP-1, TIMP-2 and TIMP-4 in stable L02 cell line with expression of pEGFP-ORF3 were down-regulated, and which provided important information for explaining the molecular mechanism through which HEV pORF3 affected the expression of protein associated with matrix metalloproteinase in target cells. |
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| Keywords: | protein chip hepatitis E L02 cell matrix metalloproteinase (MMP) expression |
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