| hrpZ_(Psta)在转基因大豆中定量表达与疫霉根腐病和灰斑病抗性相关研究 |
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| 引用本文： | 谷晓娜,刘振库,王丕武,张 卓,马 建,曲 静,张 君,付永平,卢 实,郑成忠,刘婷婷.hrpZ_(Psta)在转基因大豆中定量表达与疫霉根腐病和灰斑病抗性相关研究[J].中国油料作物学报,2015,37(1):35-40. |
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| 作者姓名： | 谷晓娜 刘振库 王丕武 张 卓 马 建 曲 静 张 君 付永平 卢 实 郑成忠 刘婷婷 |
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| 作者单位： | 1.吉林农业大学生物技术中心，吉林 长春，130118；2.吉林省长春市种子管理站，吉林 长春，130000 |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项( 2011ZX08004－004) |
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| 摘 要： | 以T5转hrpZPsta基因大豆JN29-705-15和JL30-187为材料,利用实时荧光定量PCR技术(Quantitative Real Time PCR,qRT-PCR)检测了目标基因在转基因大豆不同组织中的表达量,分别利用下胚轴侵染法和叶面喷施法鉴定疫霉根腐病抗性和灰斑病抗性,并分析目的基因表达量与疫霉根腐病和灰斑病抗性的相关性。结果表明:hrpZPsta基因在大豆的叶、茎、根、籽粒中均有表达,二个株系平均相对表达量分别为8.2/6.1、0.9/0.7、6.5/4.6和0.8/0.7;T5转hrpZPsta基因大豆抗疫霉根腐病和灰斑病能力与野生型相比均有所提高,JN29-705-15对疫霉根腐病抗性从感病提高到中抗,而JL30-187从中抗提高到抗病;hrpZPsta基因在叶中的表达量也与抗灰斑病能力呈正相关,与病情级别呈极显著负相关。试验结果初步证明了外源基因hrp ZPsta在大豆植株中的表达量与受体植株对疫霉根腐病和灰斑病抗性存在一定的相关性。
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| 关 键 词： | 大豆 hrpZPsta基因 疫霉根腐病 灰斑病 抗性 相对定量表达 |
Gene expression and disease resistance analysis of transformed hrpZPsta gene in transgenic soybeanChina |
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| GU Xiao-na,LIU Zhen-ku,WANG Pi-wu,ZHANG Zhuo,MA Jian,QU Jing,ZHANG Jun,FU Yong-ping,LU Shi,ZHENG Cheng-zhong,LIU Ting-ting.Gene expression and disease resistance analysis of transformed hrpZPsta gene in transgenic soybeanChina[J].Chinese Journal of Oil Crop Sciences,2015,37(1):35-40. |
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| Authors: | GU Xiao-na LIU Zhen-ku WANG Pi-wu ZHANG Zhuo MA Jian QU Jing ZHANG Jun FU Yong-ping LU Shi ZHENG Cheng-zhong LIU Ting-ting |
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| Institution: | GU Xiao-na;LIU Zhen-ku;WANG Pi-wu;ZHANG Zhuo;MA Jian;QU Jing;ZHANG Jun;FU Yong-ping;LU Shi;ZHENG Cheng-zhong;LIU Ting-ting;Jilin Agricultural University;Jilin Changchun Seed Control Station; |
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| Abstract: |
T5 generation of transgenic soybean-lines JN29-705-15 and JL30-187 with hrpZPsta gene were investigated by real-time quantitative polymerase chain reaction technique (qRT-PCR) to analysis the relationship between expression of the target gene in different tissues of the transgenic soybean and their resistance to Phytophthora root rot and gray leaf spot. The results showed hrpZPsta gene was expressed in soybean leaves, stems, roots and seeds, the average relative expression levels of the two strains were 8.2/6.1, 0.9/0.7, 6.5/4.6 and 0.8/0.7, respectively; the resistance to Phytophthora root rot and gray leaf spot disease were improved compared with wild-type; compared to control, the resistance against Phytophthora root rot disease of JN29-705-15 increased from sensitive to medium-resistant; and resistance of JL30-187 increased from medium to full-resistant; the expression levels of hrpZPsta gene in leaves was positively related to resistance of gray leaf spot, and significant negative correlation to the disease levels. These results indicated that the expression level of hrpZPsta was correlated to resistance to both Phyophthora root rot and gray leaf spot in transgenic soybean. |
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| Keywords: | Soybean hrpZPsta gene Phytophthora root rot disease Gray leaf spot disease Resistance Relative quantitative expression |
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