| 基于PCR检测棉花4种病原菌在新疆北部分布 |
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| 引用本文： | 萨吉达木·艾则孜,赵志强,阿孜古丽·阿不力孜,马依努尔·米吉提,郭庆元.基于PCR检测棉花4种病原菌在新疆北部分布[J].新疆农业科学,2018,55(2):293-303. |
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| 作者姓名： | 萨吉达木·艾则孜 赵志强 阿孜古丽·阿不力孜 马依努尔·米吉提 郭庆元 |
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| 作者单位： | 新疆农业大学农学院,乌鲁木齐 830052 |
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| 基金项目： | 农业部专项“作物根腐类病害防控技术研究与集成”(201503112);新疆维吾尔自治区重点研发计划“棉田主要病虫防治及农药减施增效新技术与产品研发”(2016B01001) |
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| 摘 要： | 【目的】明确新疆北部棉花主要病原菌及其分布,为新疆棉花数据库的建立提供数据,为棉花主要病害的高效防控提供依据。【方法】针对棉花主要根茎部病害从新疆北部9个市县级(含附近团场)采样区域中广泛采集病株,利用4种病害(枯萎病、立枯病、黄萎病和红腐病)各自的病原菌的特异性引物进行PCR检测。【结果】从9个采样区域,共72份以村(连)为单位的混合病样中,普遍或较普遍地检测到4种病害的病原菌,但各病原菌检出率有较大差异。其中,尖孢镰刀菌的检出率较高,为84.72%;立枯丝核菌次之,为56.94%;大丽轮枝菌和串珠镰刀菌检出率相对较低,分别为45.83%和37.50%。【结论】在所有采样区域中尖孢镰刀菌及所引起的枯萎病分布最为普遍,立枯丝核菌及所引起的立枯病次之,2种病原菌及所致病害在各采样区域的大多数村(连)都有分布;大丽轮枝菌及所引起的黄萎病在各采样区域以及近半数的村(连)有分布,串珠镰刀菌及所引起的红腐病分布不广,有明显的区域性,仅个别采样区域有较高频的分布。
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| 关 键 词： | 棉花 根茎部病害 尖孢镰刀菌 立枯丝核菌 大丽轮枝菌 串珠镰刀菌 PCR |
| 收稿时间： | 2018-01-30 |
Distribution of Four Kinds of Pathogens for Cotton Disease Based on PCR in Northern Xinjiang |
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| Sajidamu Aizezi,ZHAO Zhi-qiang,Aziguli Abulizi,Mayinuer Mijiti,GUO Qing-yuan.Distribution of Four Kinds of Pathogens for Cotton Disease Based on PCR in Northern Xinjiang[J].Xinjiang Agricultural Sciences,2018,55(2):293-303. |
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| Authors: | Sajidamu Aizezi ZHAO Zhi-qiang Aziguli Abulizi Mayinuer Mijiti GUO Qing-yuan |
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| Institution: | College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | 【Objective】 The objective of this project aims to make clear the distribution of the main pathogenic bacteria of cotton in northern Xinjiang and provide data for the establishment of cotton database, thus providing a basis for the effective prevention and control of diseases. 【Method】Four cotton main root and stem disease samples were collected from wide fields in 9 specimen collection areas of northern Xinjiang, using the pathogens specific primers of the 4 kind of main diseases (fusarium wilt, damping off, verticillium wilt red and rot) to carry out PCR detection of the diseases samples collected from northern Xinjiang. 【Result】The 4 kinds of pathogens were detected widely or more generally in the mixed samples from the 72 villages (or companies) of 9 specimen collection areas, but there were bigger difference in the detection rate among each pathogen. Among them, the detection rate of Fusarium oxysporum was higher, at 84.72%; and Rhizoctonia solani was the second, at 56.94%; while the detection rates of the Verticillium dahliae and Fusarium moniliforme were relatively low, at 45.83% and 37.50% respectively. 【Conclusion】These results showed that: F. oxysporum and fusarium wilt caused by it are the most common in all sampling areas, followed by R. solani and the disease caused by it. The two kinds of pathogens and the diseases caused by them are distributed in most villages in each sampling area; Verticillium dahliae and Verticillium dahliae are distributed in all sampling areas and nearly half of the villages. But, the distribution of F. moniliforme and red rot caused by it are not widely distributed, with obvious regional characteristics. Only higher frequency distribution is found in a few specimen collection areas. |
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| Keywords: | cotton Rhizome disease pathogen Fusarium oxysporum Rhizoctonia solani Verticillium dahliae Fusarium moniliforme polymerase chain reaction |
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