| 大晶帽石斛再生体系建立研究 |
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| 引用本文： | 吴佩珂,林蔚,李菁,王雪妮,范正阳,孙文忠,何碧珠.大晶帽石斛再生体系建立研究[J].现代农业科技,2020(9). |
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| 作者姓名： | 吴佩珂 林蔚 李菁 王雪妮 范正阳 孙文忠 何碧珠 |
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| 作者单位： | 福建农林大学,,,,,,福建农林大学 |
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| 摘 要： | 本文以成熟度70%原生大晶帽石斛荚果内种子为外植体,探究采用不同消毒时间、培养基成分构成及浓度配比对大晶帽石斛无菌萌发的影响。结果表明,大晶帽石斛荚果用5%次氯酸钠消毒15 min,可有效降低污染率;将消毒后荚果种子接入MS+蛋白胨2 g/L+NAA 0.5 mg/L+花宝1号2 g/L+0.1%活性炭+0.58%琼脂+2%蔗糖培养基中,种子萌发率达88%,是适宜大晶帽石斛无菌萌发的培养基配方。
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| 关 键 词： | 大晶帽石斛 种子 无菌萌发 培养基 消毒时间 |
| 收稿时间： | 2020/2/4 0:00:00 |
| 修稿时间： | 2020/2/4 0:00:00 |
Study on the regeneration system of Dendrobium macrocephalum |
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| Authors: | HE Bizhu |
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| Institution: | Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,Fujian Agriculture and Forestry University,Fuzhou |
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| Abstract: | The pods of Dendrobium macrocap with maturity of 70% were used as explants, the effects of different composition and concentration of medium and disinfection time on the aseptic germination of Dendrobium Dendrobium were investigated in order to explore the most suitable treatment method for the aseptic germination of Dendrobium. The results showed that Disinfect pods with 5% sodium hypochlorite for 15 minutes, It can effectively reduce the pollution rate after inoculation, adding MS+seed peptone 2g.L-1 + NAA 0.5 mg.L-1 + 2 g/L + active charcoal 0.1% + agar 0.58% + sucrose 2% was the best treatment, the seed germination rate can reach 88%. |
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| Keywords: | Dendrobium macrocephalum seeds aseptic budding medium disinfection time |
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