| 高粱花叶病毒广西甘蔗分离物全基因组序列分析 |
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| 引用本文： | 周龙武,;徐正银,;唐瑶,;吕榜丽,;李建勇,;司慧娟,;温荣辉,;陈保善.高粱花叶病毒广西甘蔗分离物全基因组序列分析[J].广西农业生物科学,2014(5):953-960. |
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| 作者姓名： | 周龙武 ;徐正银 ;唐瑶 ;吕榜丽 ;李建勇 ;司慧娟 ;温荣辉 ;陈保善 |
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| 作者单位： | [1]广西大学生命科学与技术学院,南宁530005; [2]广西大学农学院,南宁530005; [3]亚热带农业生物资源保护与利用国家重点实验室广西大学,南宁530005 |
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| 基金项目： | 广西自治区主席科技资金项目（1116602）; 广西自然科学基金项目（2013GXNSFCB019003）共同资助 |
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| 摘 要： | 近年来,高粱花叶病毒（sorghum mosaic virus,SrMV）已成为危害广西甘蔗的主要病原病毒,但尚未见侵染本地区甘蔗的SrMV全基因组序列的报道。本研究以田间一株感染SrMV的甘蔗为材料,提取叶片总RNA通过RT-PCR及RACE技术扩增获得9 626 nt（不包括polyA尾）的病毒全长基因组序列,命名为SrMV-GX。序列分析表明,SrMV-GX与浙江萧山分离物（SrMV-XoS）、浙江余杭分离物（SrMV-YH）和美国德克萨斯州分离物（SrMV-H）的核苷酸同源性分别为95.4%、94.7%和80.9%编码的多聚蛋白氨基酸同源性分别为97.8%、98.4%和90.4%。与马铃薯Y病毒科的其它成员一样,SrMV-GX基因组中存在一个小的ORF（pretty interesting potyviridae ORF,pipo）,且其序列非常保守。本研究对SrMV-GX进行序列分析,为进一步研究SrMV的遗传多样性,改进现有的检测方法及培育健康脱毒甘蔗种苗提供理论依据。
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| 关 键 词： | 高粱花叶病毒 全基因组 序列分析 pipo基因 |
Complete Genome Sequence Analysis of Sorghum Mosaic Virus from Sug- arcane in Guangxi |
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| Institution: | Zhou Longwu Xu Zhengyin Tang Yao Lv Bangli Li Jianyong Si Huijuan Wen Ronghui Chen Baoshan (1 College of Life Science and Technology fo Guangxi University, Nanning, 530005; 2 Agricultural College of Guangxi University, Nanning, 530005; 3 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530005) |
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| Abstract: | Recently, sorghum mosaic virus （SrMV） has become a major pathogenic virus in sugarcane of Guangxi province, which could constitute a serious risk to the sugarcane production. So far, there is no report of a complete genome sequence of SrMV infected sugarcane in Guangxi. The total RNA of sugarcane infected SrMV was ex- tracted and RT-PCR and RACE were used to amplify the full length genome sequence of SrMV. A 9 626 nt viral genome sequence was obtained and named SrMV-GX. Sequence analysis showed that SrMV-GX shared 95.4%, 94.7% and 80.9% nucleotide identity with Xiaoshan isolate （SrMV-XoS）, Yuhang isolate （SrMV-YIt） and Texas isolates （SrMV-H）, respectively. The deduced polyprotein of the SrMV-GX genome shared 97.8%, 98.4%, and 90.4% amino acid identity with SrMV-XoS, SrMV-YH, and SrMV-H, respectively. Just like other members ofpotyviridae, SrMV-GX genome also contained a small ORF pipo （pretty interesting potyviridae ORF）, and its sequence was highly conservative. Our results provided a theoretical basis for further studying the genetic diversity of the SrMV population, improving the diagnostic methods of SrMV, and cultivating virus-free seed cane. |
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| Keywords: | SrMV Complete genome Sequence analysis pipo |
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