| 树兰组织培养外植体消毒方法初探 |
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| 引用本文： | 易双双,陆顺教,尹俊梅,冷青云,杨光穗.树兰组织培养外植体消毒方法初探[J].广西农业生物科学,2014(4):897-901. |
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| 作者姓名： | 易双双 陆顺教 尹俊梅 冷青云 杨光穗 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室,儋州571737 |
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| 基金项目： | 948项目（2010-G2-01）; 国家国际科技合作专项项目（2011DFB31690）共同资助 |
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| 摘 要： | 以树兰（Epidendrum）为材料,分别研究了NaClO和HgCl2对树兰侧芽、茎段和花梗3种外植体的消毒效果,探讨树兰3种外植体的有效消毒方法。试验结果表明：（1）3种外植体中最易消毒的为花梗,其次是侧芽,最难消毒的为茎段;（2）HgCl2对3种外植体的消毒效果优于NaClO;（3）3种外植体最适宜的消毒方法分别为：花梗0.1%HgCl2消毒8 min,侧芽0.1%HgCl2消毒4 min,茎段0.1%HgCl2消毒10 min,其存活率分别为90.9%、42.3%和30.9%。本研究结果将为树兰外植体途径的组培再生提供理论依据和技术指导。
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| 关 键 词： | 树兰 外植体 消毒方法 组织培养 |
The Preliminary Study in Explants Disinfection Method of Epidendrum in Tissue Culture |
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| Yi Shuangshuang,Lu Shunjiao,Yin Junmei,Leng Qingyun,Yang Guangsui.The Preliminary Study in Explants Disinfection Method of Epidendrum in Tissue Culture[J].Journal of Guangxi Agricultural and Biological Science,2014(4):897-901. |
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| Authors: | Yi Shuangshuang Lu Shunjiao Yin Junmei Leng Qingyun Yang Guangsui |
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| Institution: | ( Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou, 571737) |
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| Abstract: | To study the effective sterilization method of Epidendrum, the disinfection effect of NaClO and HgC12 for Epidendrum lateral bud, stem and flower stalk were analyzed respectively. The results showed that： （1） Flower stalk was the most easily disinfected among the three explants, then was lateral bud, the other was stem. （2） The disinfection effect of HgCl2 was apparently better than NaClO. （3） The optimum sterilization method of flower stalk, lateral bud and stem of Epide ndrum was disinfected with 0.1% HgCl2 for 8 min, 4 min and 10 min, respectively, and the survival rate accordingly reached up to 90.9%, 42.3% and 30.9%. This result will provide theoretical basis and technical guidance for tissue culture of Epidendrum. |
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| Keywords: | Epidendrum Explant Disinfection method Tissue culture |
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