| 十字花科黑腐病菌中一个与黄色素合成相关基因的鉴定 |
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| 引用本文： | 杨国奎,;祁艳华,;朱艳宁,;冷明,;叶玉云,;苏辉昭,;陆光涛.十字花科黑腐病菌中一个与黄色素合成相关基因的鉴定[J].广西农业生物科学,2014(4):802-807. |
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| 作者姓名： | 杨国奎 ;祁艳华 ;朱艳宁 ;冷明 ;叶玉云 ;苏辉昭 ;陆光涛 |
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| 作者单位： | [1]广西大学生命科学与技术学院,南宁530005; [2]亚热带农业资源保护与利用国家重点实验室,南宁530005 |
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| 基金项目： | 国家自然科学基金（31171206）项目资助
致谢 本研究是在国家自然科学基金（31171206）项目的支持下完成,衷心感谢参与本研究的全体老师和所有同学. |
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| 摘 要： | 十字花科黑腐病菌（Xanthomonas campestris pv.campestris,简称Xcc）能够产生大量的胞外多糖。胞外多糖商品名又称为黄原胶,具有广泛用途,在食品生产中可作为添加剂使用。为了得到不含黄色素的黄原胶,我们采用转座子EZ-Tn5随机插入诱变的方法对8004菌株进行突变,获得了一株不产黄色素的突变体。通过对突变体的转座子EZ-Tn5插入位点进行分析,发现该突变体是由于编号为XC_4097的基因被插入突变后衍生而来。同源性分析表明XC_4097编码一种脂质酰基转移酶,它与脂质的合成有关。采用同源双交换方法构建XC_4097基因的缺失突变体。通过对野生菌、突变体以及功能回补体的表型分析进一步证实了XC_4097基因功能的丧失只影响黑腐病菌黄色素的合成,而不影响细菌生长以及胞外多糖的合成。这为工业上生产无黄色素的黄原胶提供了应用基础。
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| 关 键 词： | 十字花科黑腐病菌 胞外多糖 黄色素 XC_4097 |
Identification of a Gene Involved in Pigment Synthesis in Xanthomonas campestris pv. campestris |
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| Institution: | Yang Guokui,Qi Yanhua,Zhu Yanning,Leng Ming,Ye Yuyun,Su Huizhao,Lu Guangtao(1 College of Life Science and Technology, Guangxi University, Nanning, 530005; 2 State Key Laboratory of Conservation and Utilization of Sub- tropical Agro-bioresources, Nanning, 530005) |
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| Abstract: | Xanthomonas campestris pv. campestris（Xcc） produces a mass of exopolysaccharides, which are also called xanthan gum. Xanthan gum could be used in a variety of products, including used as additive agents in food production. In order to attain the non-pigment xanthan gum, Xcc8004 strain was mutanted by using transposon EZ-Tn5 and a mutant strain producing no pigment was isolated. Further analysis revealed that the mutant is derived from the insertion of EZ-Tn5 in XC_4097 in the genome of 8004 strain. Sequence analysis showed that this gene is predicted to encode a lipid acyl transferase, which is involved in lipid synthesis. The XC_4097 deletion mutant was constructed by the method of homologous double-crossover. Through analyzing phenotype of wild type, mutant and complementary strains demonstrate that the XC_4097 involved in pigment synthesis but not exopolysaccharides（EPS） yield and growth in Xcc. This work provides foundation for producing xanthan gum without pigment. |
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| Keywords: | Xanthomonas campestris pv campestris Exopolysaccharides Pigment XC_4097 |
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