| 水稻抗病基因同源序列多态性与品种鉴定 |
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| 引用本文： | 孙雁,王云月,何月秋,朱有勇.水稻抗病基因同源序列多态性与品种鉴定[J].种子,2001(2):1-2. |
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| 作者姓名： | 孙雁 王云月 何月秋 朱有勇 |
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| 作者单位： | 孙雁（云南省植物病理重点实验室,昆明,650201）
王云月（云南省植物病理重点实验室,昆明,650201）
何月秋（云南省植物病理重点实验室,昆明,650201）
朱有勇（云南省植物病理重点实验室,昆明,650201） |
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| 基金项目： | 国家863项目(Z16-03-M)、云南省科技攻关项目和国际水稻所合作项目(IRRI-ADP37)资助 |
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| 摘 要： | 利用已知植物抗病基因不同保守序列设计的S1/AS3和XLRR for/XLRR rev两对引物对22个水稻品种DNA进行PCR扩增和聚丙烯酰胺凝胶电泳.分析结果表明水稻抗病基因同源序列类型丰富,2对引物共扩增出143条带,品种间有差异的谱带92条,根据谱带差异可将供试品种完全鉴别出来.证明抗病基因同源序列分析可以用于水稻品种鉴定.
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| 关 键 词： | 水稻 抗病基因同源序列 多态性 品种鉴定 |
Genetic Diversity and Identification among Rice Varieties Based on Resistance Gene Analogues |
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| Sun Yan,Wang Yunyue,He Yueqiu,Zhu Youyong.Genetic Diversity and Identification among Rice Varieties Based on Resistance Gene Analogues[J].Seed,2001(2):1-2. |
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| Authors: | Sun Yan Wang Yunyue He Yueqiu Zhu Youyong |
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| Abstract: | The genetic diversity of 22 rice commercial and local resource varieties in Yunnan Province were studied using resistance gene analogue (RGA) based on different resistance gene conserved sequences. The RGA analysis was proceeded by the PCR amplification using the primers: S1/AS3 designed from the RPS2 and N gene sequences of Arabidopsis thaliana and tobacco, and XLRR for/ XLRR rev designed based on the resistance gene Xa21 for Xanthomonas oryzae pv oryzae, respectively. The results showed that both primer pairs were identified with polymorphism, which produced 92 polymorphic alleles totally. All the varieties were genetically highly diverse and completely distinguished from their patterns. The present technique might offer another promising approach for identifying rice varieties. |
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| Keywords: | rice resistance gene analogue polymorphism variety identification |
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