| 混合病毒在不同宿主中交替繁殖对病毒毒力的影响 |
| |
| 引用本文： | 郭慧芳,韩召军,方继朝,钟万芳,王节萍,刘宝生.混合病毒在不同宿主中交替繁殖对病毒毒力的影响[J].农药学学报,2005,7(3):242-248. |
| |
| 作者姓名： | 郭慧芳 韩召军 方继朝 钟万芳 王节萍 刘宝生 |
| |
| 作者单位： | 南京农业大学,昆虫学系,江苏,南京,210095;江苏省农业科学院,植物保护研究所,江苏,南京,210014;南京农业大学,昆虫学系,江苏,南京,210095;江苏省农业科学院,植物保护研究所,江苏,南京,210014 |
| |
| 基金项目： | 国家“十五”科技攻关项目（2002BA516A）;江苏省自然科学基金重点项目（BK2002207）;江苏省农业科学院基金项目（6110542）. |
| |
| 摘 要： | 研究了混合病毒在不同宿主中交替繁殖对病毒毒力的影响,选用对甜菜夜蛾Spodoptera exigua (Hüber)较专化的苜蓿银纹夜蛾核多角体病毒(AcNPV)和对斜纹夜蛾Spodoptera litura (Fabricius) 较专化的斜纹夜蛾核多角体病毒(SlNPV),按多角体数量1∶ 1混合后分别连续感染或交替感染甜菜夜蛾和斜纹夜蛾,比较各代病毒对两种夜蛾的活性。测定结果表明:SlNPV中国株和AcNPV混合,起始感染甜菜夜蛾,再在斜纹夜蛾和甜菜夜蛾中交替繁殖后,F4代斜纹夜蛾病毒(由斜纹夜蛾中收集的病毒)对3龄甜菜夜蛾幼虫的致死率极显著高于F2代斜纹夜蛾病毒,F3代甜菜夜蛾病毒(由甜菜夜蛾中收集的病毒)对斜纹夜蛾的致死率也显著高于F1代甜菜夜蛾病毒;混合病毒起始感染斜纹夜蛾,再经甜菜夜蛾和斜纹夜蛾交替繁殖后,斜纹夜蛾病毒对甜菜夜蛾的毒力以及甜菜夜蛾病毒对斜纹夜蛾的毒力也都显著提高。交替传代后的病毒比同代次连续传代后的病毒对非宿主的毒力显著提高,并且它们对原宿主的毒力未有减弱。AcNPV和SlNPV日本株混合病毒在甜菜夜蛾和斜纹夜蛾中交替繁殖后也表现出对非宿主毒力的提高。表明混合病毒在两种不同原宿主中交替繁殖比其在同种宿主中连续繁殖后对非繁殖原宿主的毒力显著提高。
|
| 关 键 词： | 苜蓿银纹夜蛾核多角体病毒 斜纹夜蛾核多角体病毒 交替繁殖 毒力 斜纹夜蛾 甜菜夜蛾 |
| 文章编号： | 1008-7303（2005）03-0242-07 |
| 收稿时间： | 2005-03-02 |
| 修稿时间： | 2005-04-26 |
Changes in the Virulence of Mixed Nucleopolyhedroviruses when Propagated in Two Noctuid (Lepidoptera: Noctuidae) Hosts Alternately |
| |
| GUO Hui-fang,HAN Zhao-jun,FANG Ji-chao,ZHONG Wan-fang,WANG Jie-ping and LIU Bao-sheng.Changes in the Virulence of Mixed Nucleopolyhedroviruses when Propagated in Two Noctuid (Lepidoptera: Noctuidae) Hosts Alternately [J].Chinese Journal of Pesticide Science,2005,7(3):242-248. |
| |
| Authors: | GUO Hui-fang HAN Zhao-jun FANG Ji-chao ZHONG Wan-fang WANG Jie-ping and LIU Bao-sheng |
| |
| Institution: | 1.Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China2.Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China |
| |
| Abstract: | The effect of alternated propagation of nucleopolyhedroviruses(NPV) in two insect hosts on virulence was firstly investigated. The Spodoptera litura NPV(SlNPV)and Autographa californiaNPV (AcNPV) were mixed equally to infect S. exigua and S. litura, respectively, and after that, they were propagated in the original host successively or in S. exigua and S. litura alternately. And the (virulence) of NPVs of each generation were compared. The results showed that alternated propagation in two different hosts significantly improved the virulence of NPV. NPV propagated in S. litura were more effective against S. exigua after one passage in S. (exigua, )and its efficacy on S. litura did not (decrease.) And it was the same to NPV propagated in S. exigua againt S. litura. NPV propagated in (S. exigua) was also more effective against S. litura after one passage in S. litura, and its efficacy on S. exigua did not decrease. All the results indicated that, compared to the efficacy of NPV after serial (passages) in same host, the efficacy of NPV against non-host target was improved after alternated propagation of mixed NPVs in two different hosts. |
| |
| Keywords: | AcNPV SlNPV alternated propagation virulence Spodoptera litura Spodoptera exigua |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《农药学学报》浏览原始摘要信息 |
| 点击此处可从《农药学学报》下载免费的PDF全文 |
|
