| 安徽省小麦孢囊线虫的发生分布与鉴定 |
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| 引用本文： | 陶玲,吴慧平,彭德良,吴向晖,张胜.安徽省小麦孢囊线虫的发生分布与鉴定[J].安徽农业大学学报,2012,39(2):257-262. |
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| 作者姓名： | 陶玲 吴慧平 彭德良 吴向晖 张胜 |
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| 作者单位： | 安徽农业大学植物保护学院,合肥,230036;中国农业科学院植物保护研究所,北京,100193;安徽省植保总站,合肥,230036 |
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| 基金项目： | 国家公益性行业(农业)科研专项(200903040)资助 |
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| 摘 要： | 为了解小麦孢囊线虫(Cereal cyst nematode,CCN)在安徽省的分布和主要麦区CCN的种类,以及群体间关系,调查了淮北等麦区小麦根系白雌虫,用"Zig-Zag"法在怀远等地采集土样,用Fenwick&Oostenbrink法分离土样中的孢囊并孵化得2龄幼虫,利用孢囊阴门锥形态及de-man法鉴定CCN种类,扩增ITS特征片段,用MEGA5.05构建系统发育树。结果表明,安徽省52%采样点检出CCN,淮河以北采样点均检出CCN,江淮麦区7%的采样点检出CCN,长江以南麦区采样点未检出CCN。发生在怀远、凤台、灵璧、界首和颍上麦区小麦根际CCN鉴定为禾谷孢囊线虫(Heterodera avenae),淮河以北发生H.avenae麦区占淮北小麦种植面积的41.6%,另外,怀远H.avenae群体和其他群体亲缘关系较远,群体间形态有一定差异,但和亲缘关系差异无一致性。
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| 关 键 词： | 小麦孢囊线虫 分布 群体 鉴定 |
Distribution and identification of cereal cyst nematodes on wheat in Anhui Province, China |
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| TAO Ling,WU Hui-ping,PENG De-liang,WU Xiang-hui and ZHANG Sheng.Distribution and identification of cereal cyst nematodes on wheat in Anhui Province, China[J].Journal of Anhui Agricultural University,2012,39(2):257-262. |
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| Authors: | TAO Ling WU Hui-ping PENG De-liang WU Xiang-hui and ZHANG Sheng |
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| Institution: | 1(1.School of Plant Protection,Anhui Agricultural University,Hefei 230036; 2.Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193; 3.Plant Protection Station of Anhui Province,Hefei 230036) |
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| Abstract: | The purpose of this paper is to survey distribution,species of cereal cyst nematode(CCN) and re-lationship among CCN species in Anhui Province.White female of wheat root system in Huaibei wheat areas were investigated;soil samples of wheat rhizosphere were collected with "Zig-Zag" method in Huaiyuan county and other counties;cysts were separated by Fenwick & Oostenbrink method from those samples;second-stage juveniles(J2) were obtained by hatching cyst;CCN was identified based on morphologic characteristics of cyst perinea pattern and de-man data;and the genetic relationships were analyzed by amplified ITS characteristic fragment and phylogenetic tree built with MEGA5.05.The results showed that CCN was detected from 52% sam-ple sites,from all the samples of wheat areas in the northern part of the Huai River,and from 7% sample sites in Jianghuai wheat areas.No CCN was detected from wheat areas in the southern part of the Changjiang River.Oc-currence of CCN in Yingshang and other 4 counties were identified as Heterodera avenae,and occurrence area of H.avenae was up to 41.6% of wheat areas in the northern part of the Huai River.Huaiyuan population of H.avenae is distantly related with other 4 H.avenae populations,and certain morphological differences exist among the 5 H.avenae populations,but with no consistency to the genetic relationships. |
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| Keywords: | Heterodera avenae distribution population identification |
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