| 不同方法提取吴茱萸叶片基因组DNA和RNA的比较 |
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| 引用本文： | 吴波,高丹,潘超美,张寿文.不同方法提取吴茱萸叶片基因组DNA和RNA的比较[J].安徽农业大学学报,2012,39(1):111-115. |
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| 作者姓名： | 吴波 高丹 潘超美 张寿文 |
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| 作者单位： | 广州中医药大学中药学院,广州510006;江西中医学院江西省中药种质资源工程技术研究中心,南昌330004;江西中医学院药学院,南昌,330004;广州中医药大学中药学院,广州,510006 |
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| 基金项目： | 国家“十二五”科技支撑计划项目(2011BAI04B04)资助 |
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| 摘 要： | 分别研究对比了3种不同方法提取吴茱萸叶片基因组DNA和RNA的质量,以确定适合吴茱萸叶片基因组DNA和RNA的提取方法。采用紫外分光光度法和普通电泳检测法比较了常规CTAB法、SDS法和改良CTAB法提取吴茱萸叶片基因组DNA的效果;采用紫外分光光度法和非变性电泳检测法比较了酚-SDS法、Trizol法和改良异硫氰酸胍法提取吴茱萸叶片RNA的效果。结果表明,分别采用不同方法提取的吴茱萸叶片基因组DNA和RNA的质量差异较大。常规CTAB法和SDS法无法提取出高质量的吴茱萸叶片基因组DNA,而改良CTAB法提取效果较好;酚-SDS法和Trizol法不适合高质量吴茱萸叶片RNA的提取,而改良异硫氰酸胍法的提取效果良好。
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| 关 键 词： | 吴茱萸 基因组DNA RNA 提取方法 |
Comparation on different methods on genomic DNA and RNA extraction from young leaves of Evodia rutaecarpa |
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| WU Bo,GAO Dan,PAN Chao-mei and ZHANG Shou-wen.Comparation on different methods on genomic DNA and RNA extraction from young leaves of Evodia rutaecarpa[J].Journal of Anhui Agricultural University,2012,39(1):111-115. |
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| Authors: | WU Bo GAO Dan PAN Chao-mei and ZHANG Shou-wen |
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| Institution: | 1.College of Pharmacy,Guangzhou University of Chinese Medicine,Guangzhou 510006; 2.Chinese Medicine Germplasm Resource Engineering Technology Research Center,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004; 3.College of Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004) |
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| Abstract: | In order to definite suitable extraction methods of genomic DNA and RNA from Evodia rutae-carpa,s young leaves,three difference methods were compared each other,respectively.Ultraviolet spectropho-tometry and ordinary electrophoresis methods were used to detect and compare the extraction effect of genomic DNA on traditional CTAB method,SDS method and modified CTAB method.Ultraviolet spectrophotometry and native gel electrophoresis were used to detect and compare the extraction effect of RNA on phenol-SDS method,Trizol method and modified guanidine thiocyanate method.The result showed that the qualities of genomic DNA and RNA extracted from Evodia rutaecarpa,s young leaves were clearly different using different extraction methods.The quality of genomic DNA exacted from Evodia rutaecarpa,s young leaves with CTAB method and SDS method were not satisfied,but the extraction effect was very well using modified CTAB method.The quali-ties of RNA extracted from Evodia rutaecarpa,s young leaves with Phenol-SDS method and Trizol method were not high,but the extraction effect was very well using modified guanidine thiocyanate method. |
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| Keywords: | Evodia rutaecarpa genomic DNA RNA extraction method |
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