| 比色法测定菠菜中草酸含量的条件研究 |
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| 引用本文： | 段立珍,汪建飞,赵建荣.比色法测定菠菜中草酸含量的条件研究[J].安徽农业科学,2007,35(3):632-633,643. |
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| 作者姓名： | 段立珍 汪建飞 赵建荣 |
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| 作者单位： | 安徽科技学院资源环境系,安徽凤阳,233100;安徽科技学院资源环境系,安徽凤阳,233100;安徽科技学院资源环境系,安徽凤阳,233100 |
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| 基金项目： | 安徽省自然科学基金项目（050410105）：安徽省高校科研基金项目（2005KJ153）. |
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| 摘 要： | 对水杨酸铁络合比色法测定菠菜中草酸含量的条件进行了研究,结果表明,最佳条件是:显色体系pH值2.1～2.7,比色波长510nm,比色时间20 min～2 h.该法测定菠菜样品中草酸含量范围在0～64.0 μg/ml,检出限为1.52 μg/ml;样品加标回收率93.9%～105.2%.
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| 关 键 词： | 比色法 草酸 磺基水杨酸络合物 菠菜 |
| 文章编号： | 0517-6611（2007）03-00632-02 |
| 修稿时间： | 2006-10-17 |
Study on the Determining Conditions of Oxalate Content in Spinach with Coloration Method |
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| DUAN Li-zhen et al.Study on the Determining Conditions of Oxalate Content in Spinach with Coloration Method[J].Journal of Anhui Agricultural Sciences,2007,35(3):632-633,643. |
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| Authors: | DUAN Li-zhen |
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| Institution: | Anhui Science and Technology University, Fengyang, Anhui 233100 |
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| Abstract: | The study on the determining conditions of oxalate content in spinach indicated that the optimum conditions were that the pH was the range of 2.1～2.7, the coloration wavelength was 510 nm,and the suitable coloration time was 20 min～2 h.The oxalate content range of determination with the method was 0～64.0 μg/ml,the detection limit was 1.52 μg/ml and the recovery rate was 93.9%～105.2%. |
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| Keywords: | Spectrophotometry coloration method Oxalate Sulfosalicylic acidic complex Spinach |
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