| 不同成熟时间的猪卵母细胞对体外受精的影响及孤雌发育 |
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| 引用本文： | 张菁,朱淑文,华修国,李华威.不同成熟时间的猪卵母细胞对体外受精的影响及孤雌发育[J].猪业科学,2004,21(4):40-42. |
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| 作者姓名： | 张菁 朱淑文 华修国 李华威 |
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| 作者单位： | 上海交通大学农业与生物学院动物科学系,上海,201101;上海交通大学农业与生物学院动物科学系,上海,201101;上海交通大学农业与生物学院动物科学系,上海,201101;上海交通大学农业与生物学院动物科学系,上海,201101 |
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| 基金项目： | 上海市科委国际合作项目(015407005) |
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| 摘 要： | 采用NCSU-37为体外成熟、受精、培养体系,比较不同成熟时间46h、58h和70h的猪卵母细胞对体外受精的影响和孤雌发育。结果显示,猪卵母细胞在体外成熟培养46h、58h和70h后,46h培养组的卵母细胞体外受精后的卵裂率明显低于58h和70h培养组(P<0.05),但囊胚发育率明显高于其他两组(P<0.05)。46h组的孤雌发育率明显高于其他两组(P<0.05),囊胚发育率三组之间无显著差异。表明猪卵母细胞体外成熟培养时间延长,体外受精后的囊胚发育率降低,孤雌发育率相应增加。
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| 关 键 词： | 猪 卵母细胞 体外成熟 卵裂 囊胚 孤雌发育 |
| 文章编号： | 1008-9381(2004)04-0040-03 |
| 修稿时间： | 2004年2月13日 |
Effect of Different Maturation Time on the in Vitro Fertilization and Parthenogenesis of Porcine Oocytes |
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| Zhang jing,Zhu Shu-wen,Hua Xiu-guo,Li Hua-wei.Effect of Different Maturation Time on the in Vitro Fertilization and Parthenogenesis of Porcine Oocytes[J].Swine Industry Science,2004,21(4):40-42. |
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| Authors: | Zhang jing Zhu Shu-wen Hua Xiu-guo Li Hua-wei |
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| Abstract: | Porcine oocytes were cultured in NCSU-37,compared effect of in vitro fertilization and parthenogenesis on porcine oocytes in different maturation time (46 h?58 h or 70 h), At 48h after insemination, the cleavage rates in the 46 h group was significantly lower than in 58 h and 70 h groups(P<0.05),but the blastocyst rates 46 h group was significantly higher than thant groups(P<0.05). The parthenogenesis rates in the 46 h group was significantly lower than that in the 58 h and 70 h groups(P<0.05). It appears that the blastocyst rates would decline with length the maturation time of porcine oocytes in vitro, but their parthenogenesis would increase. |
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| Keywords: | porcine oocyte in vitro maturation cleavage blastocyst parthenogenesis |
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