| HPLC法检测哈巴苷及其黑曲霉转化产物 |
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| 引用本文： | 朱露娟,胡娜,周斌,常军.HPLC法检测哈巴苷及其黑曲霉转化产物[J].安徽农业科学,2009,37(36). |
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| 作者姓名： | 朱露娟 胡娜 周斌 常军 |
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| 作者单位： | 江西科技师范学院生命科学学院,江西南昌,330013;江西科技师范学院药学院,江西南昌,330013 |
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| 摘 要： | 目的]研究黑曲霉转化液中哈巴苷及其3个转化产物的同时检测方法。方法]通过用HPLC-SPE法快速测定黑曲霉转化液中哈巴苷及其3个转化产物的含量考察了该法的应用条件。结果]哈巴苷、哈巴苷代谢素Ⅰ、哈巴苷代谢素Ⅱ和桃叶珊瑚甙B的线性回归方程分别为y=52.32x-89.76、y=32.52x-45.26、y=14.73x-25.64和y=41.25x-33.71,最低检测浓度分别为0.020、0.010、0.015和0.015μg/ml。0.010、0.100和2.500μg/ml哈巴苷的回收率分别为103.0%、99.7%和99.9%,0.010、0.500和2.500μg/ml的哈巴苷代谢素Ⅰ的回收率分别为102.1%、99.8%和99.9%,0.015、0.500和2.500μg/ml的哈巴苷代谢素Ⅱ的回收率分别为99.3%、99.6%和99.6%,0.015、0.500和2.500μg/ml的桃叶珊瑚甙B的回收率分别为100.6%、100.7%和99.9%。该法检测哈巴苷、哈巴苷代谢素Ⅰ、哈巴苷代谢素Ⅱ和桃叶珊瑚甙B的相对标准偏差在1.3%～4.7%。结论]该方法能够快速、高效地检测黑曲霉转化液中哈巴苷及其转化产物的含量。
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| 关 键 词： | HPLC 哈巴苷 哈巴苷代谢素Ⅰ 哈巴苷代谢素Ⅱ 桃叶珊瑚甙B |
Determination on Harpagide and Its Transformed Products of Aspergillus niger by HPLC Method |
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| ZHU Lu-juan et al.Determination on Harpagide and Its Transformed Products of Aspergillus niger by HPLC Method[J].Journal of Anhui Agricultural Sciences,2009,37(36). |
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| Authors: | ZHU Lu-juan |
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| Institution: | ZHU Lu-juan et al(College of Life Science,Jiangxi Science , Technology Normal University,Nanchang,Jiangxi 330013) |
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| Abstract: | Objective]The purpose was to study the simultaneous determination method of harpagide and its 3 transformed products in the transformation liquid of Aspergillus nige. Method]The application conditions of HPLC-SPE method were investigated through determining the contents of harpagide and its 3 transformed products in the transformation liquid of A. niger rapidly by this method. Result] The linear regression equations of harpagide, harpagidemetabolineⅠ, harpagidemetaboline Ⅱ and aucubin B were y= 52.32x-89.76, y=32.52x-45.26, y=14.73x-25.64 and y=41.25x-33.71 resp. Their minimum detectable concn. were 0.020, 0.010, 0.015 and 0.015 μg/ml resp. The recoveries of 0.010, 0.100 and 2.500 μg/ml harpagide were 103.0%, 99.7% and 99.9% resp., that of 0.010, 0.500 and 2.500 μg/ml harpagidemetabolineⅠ were 102.1%, 99.8% and 99.9% resp., that of 0.015, 0.500 and 2.500 μg/ml harpagidemetaboline Ⅱ were 99.3%, 99.6% and 99.6% resp. and that of 0.015, 0.500 and 2.500 μg/ml aucubin B were 100.6%, 100.7% and 99.9% resp. The RSDs of determination on harpagide, harpagidemetabolineⅠ, harpagidemetaboline Ⅱ and aucubin B by this method were in 1.3%～4.7%. Conclusion] The contents of harpagide and its transformed products in the transformation liquid of A. niger could be determined rapidly and efficiently by this method. |
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| Keywords: | HPLC HPLC Harpagide Aucubin B |
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