| 用农杆菌Ri诱导金银花发根培养的研究 |
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| 引用本文： | 何钢,周再魁,袁靖,刘贤桂.用农杆菌Ri诱导金银花发根培养的研究[J].中南林业科技大学学报,2008,28(5). |
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| 作者姓名： | 何钢 周再魁 袁靖 刘贤桂 |
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| 作者单位： | 中南林业科技大学生物环境科学与技术研究所，中南林业科技大学生命科学与技术学院，中南林业科技大学资源与环境学院 |
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| 基金项目： | 湖南省科技攻关计划
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| 摘 要： | 通过预培养时间、菌液稀释倍数、浸染时间、共培养时间、抗生素质量浓度5因素4水平的正交实验,得出优化组合,认为组培苗叶片外植体预培养5 d,菌液稀释45倍,浸染4 min,共培养3 d,然后转入含500 mg/L羧苄青霉素的1/2M S培养基上培养,金银花的发根诱导率最高,达54.3%.随机抽取离体培养的发根经PCR检测,证实为转化发状根.
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| 关 键 词： | 金银花 发根 发根农杆菌 诱导率 |
A Study of Hairy Roots Culture of Flos lonicerae Induced by Agrobacterium rhizogenes Ri |
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| HE Gang,ZHOU Zai-kui,YUAN Jing,LIU Xian-gui.A Study of Hairy Roots Culture of Flos lonicerae Induced by Agrobacterium rhizogenes Ri[J].Journal of Central South Forestry University,2008,28(5). |
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| Authors: | HE Gang ZHOU Zai-kui YUAN Jing LIU Xian-gui |
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| Abstract: | Orthogonal tests were conducted,on four levels,over five factors,namely,pre-culture time,tested strain concentration,infection time,co-culture time and antibiotic concentration to obtain an optimum formulation for inducing Flos.Lonicera hairy roots.Results show that the induction rate of hairy roots is up to 54.3% when the OD600 of R1000 is about 0.6~0.8,the axenic plantlet leaves are pre-cultured for 5 days,and the Agrobacterium solution,immersed 4 mins,is diluted to 45 times,co-cultured for 3 days and transferred to culture in 1/2MS medium with 500 mg/L Carb.Hairy roots taken at random from the in vitro culture were tested by PCR to be transgenic. |
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| Keywords: | Flos lonicera hairy roots Agrobacterium rhizogenes frequency of hairy roots induction |
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