| QuEChERS结合UPLC-MS/MS法测定番茄中吡蚜酮和吡丙醚残留量 |
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| 引用本文： | 王小飞,张仙,彭茂民,夏虹.QuEChERS结合UPLC-MS/MS法测定番茄中吡蚜酮和吡丙醚残留量[J].湖北农业科学,2016(24). |
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| 作者姓名： | 王小飞 张仙 彭茂民 夏虹 |
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| 作者单位： | 农业部食品质量监督检验测试中心 武汉,武汉,430064 |
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| 基金项目： | 农业部农药检定所农药登记残留试验项目 |
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| 摘 要： | 建立QuEChERS结合超高效液相色谱串联质谱(UPLC-MS/MS)法测定了番茄中吡蚜酮和吡丙醚残留量的分析方法。样品用乙腈超声提取,QuEChERS法提取和净化,UPLC-MS/MS分析检测。结果表明,在0.005~0.5 mg/L范围内,吡蚜酮的相关系数为0.994 5,吡丙醚的相关系数为0.999 6,该方法的线性关系良好。在0.02、0.2、1.0 mg/kg 3个添加浓度下,吡蚜酮的平均添加回收率为87.5%~106.1%,RSD值在2.2%~3.1%之间;吡丙醚的平均添加回收率为82.2%~89.1%,RSD值在1.6%~14.7%之间。吡蚜酮的检出限为1.0×10~(-3) mg/kg,吡丙醚的检出限为2.0×10~(-4) mg/kg。该方法适用于番茄中吡蚜酮和吡丙醚残留量的测定。
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| 关 键 词： | 超高效液相色谱串联质谱(UPLC-MS/MS) QuEChERS 番茄 吡蚜酮 吡丙醚 |
Determination of Pymetrozine and Pyriproxyfen Residues in Tomato by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| Abstract: | A method of QuEChERS combined with u1tra performance 1iquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was deve1oped for the determination of pymetrozine and pyriproxyfen residues in tomato. The tomato samp1es were extracted with acetonitri1e by u1trasonic wave, extracted purified by QuEChERS method and ana1yzed by UPLC-MS/MS. The 1inear ranges of pymetrozine and pyriproxyfen were 0.005~0.5 mg/L, and the corre1ation coefficient of pymetrozine was 0.994 5, and pyriproxyfen was 0.999 6. When the spiked 1eve1s were 0.02,0.2 and 1.0 mg/kg,the average recoveries of pymetrozine ranged from 87.5% to 106.1% with the re1ative standard deviation (RSD) from 2.2% to 3.1%,and the average recoveries of pyriproxyfen ranged from 82.2% to 89.1% with the re1ative standard deviation(RSD) from 1.6% to 14.7%. The 1imit of detec-tion (LOD) of pymetrozine was 1.0×10-3 mg/kg, and pyriproxyfen was 2.0×10-4 mg/kg. The method can be used for determi-nation of pymetrozine and pyriproxyfen residues in tomato. |
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| Keywords: | u1tra performance 1iquid chromatography-tandem mass spectrometry QuEChERS tomato pymetrozine pyriproxyfen |
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