| 枯草芽孢杆菌H4培养条件的优化 |
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| 引用本文： | 李亚玲,赵玉洁,谢凤行,周可,张峰峰.枯草芽孢杆菌H4培养条件的优化[J].天津农业科学,2009,15(4):20-23. |
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| 作者姓名： | 李亚玲 赵玉洁 谢凤行 周可 张峰峰 |
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| 作者单位： | 天津市农业生物技术研究中心,天津,300192 |
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| 基金项目： | 天津市应用基础与前沿计划项目 |
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| 摘 要： | 以枯草芽孢杆菌H4为试验材料,利用Plackett-Burman设计法,对影响菌体生长的7个因素进行了筛选,确定影响该菌生长的主要因素为牛肉膏、转速、蛋白胨和NaCl;采用响应面分析法对其中3个重要因子的最佳水平进行研究。结果表明,当牛肉膏为8.635g/L,蛋白胨为13.737g/L,NaCl为5.345g/L,接种量6.25%,初始pH7,温度39℃,转速150r/min培养时,菌体密度显著提高,菌株H4的OD460值由0.899提高到了1.129。
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| 关 键 词： | 枯草芽孢杆菌 Plackett-Burman设计 响应面分析法 优化 |
Optimization of Growth Conditions of Bacillus Subtilis H4 |
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| LI Ya-ling,ZHAO Yu-jie,XIE Feng-xing,ZHOU Ke,ZHANG Feng-feng.Optimization of Growth Conditions of Bacillus Subtilis H4[J].Tianjin Agricultural Sciences,2009,15(4):20-23. |
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| Authors: | LI Ya-ling ZHAO Yu-jie XIE Feng-xing ZHOU Ke ZHANG Feng-feng |
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| Institution: | (Tianjin Research Center of Agriculture Biotechnology, Tianjin 300192, China) |
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| Abstract: | A strain of Bacillus subtilis H4 was isolated from the mud of pound, and the Placken-Burman design was used to study the 7 factors influencing the growth of Bacillus subtilis H4. The results showed that beef extract, rotational speed, peptone and NaCl were the main factors. Response surface methodology was also used to research the optimization for the 3 main factors, and the results indicated that the concentration of Bacillus subtilis H4 was significantly increased and the OD460 of the broth increased from 0.899 to 1.129 under the condition of beef extract 8.635 g/L, peptone 13.737 g/L, NaCl 5.345 g/L, inoculation 6.25%, initial pH7, temperature 39 ℃ and rotational speed 150 r/min. |
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| Keywords: | Bacillus subtilis Plackett-Burman design response surface method optimization |
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