Molecular and Pathotype Analysis of the Rice Blast Fungus in North Vietnam |
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| Authors: | Nguyen Thi Ninh Thuan Joseph Bigirimana Ed Roumen Dominique Van Der Straeten Monica Höfte |
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| Institution: | (1) Laboratory of Phytopathology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium;(2) Institute for Plant Biotechnology for Developing Countries, Gent, Belgium;(3) Bayer CropScience, Technologiepark 38, B-9052 Gent, Belgium;(4) Department of Molecular Genetics, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium |
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| Abstract: | Rice blast caused by Pyricularia oryzae is a devastating disease worldwide. In Vietnam, rice blast is especially severe in the Red River Delta in the North. The
genetic diversity of 114 P. oryzae isolates collected from rice in 2001 in the Red River Delta and nine additional Vietnamese P. oryzae isolates was analysed using Amplified Fragment Length Polymorphism (AFLP). DNA similarity and cluster analysis based on 160
polymorphic AFLP markers showed twelve different AFLP genetic groups among the 123 field isolates. Isolates collected from
japonica hosts clustered separately from indica host isolates with at least 60% dissimilarity with little evidence for gene flow between the two populations. In the 2001
population originating from indica hosts, three genetic groups were predominant and represented 99% of the isolates sampled. One predominant clonal lineage
represented 59% of the 2001 indica host population and was found in eleven provinces in the Red River Delta of North Vietnam. Significant genotype flow could
be demonstrated between the indica population south of Red river and the indica population north of Red river. There was significant linkage disequilibrium between the AFLP loci within the indica population, indicating that this is not a random mating population. Pathogenicity tests of 25 isolates selected from the
12 AFLP groups on a set of 29 differential rice lines revealed two avirulent isolates and 23 pathotypes. Different combinations
of known resistance genes were found to have potential for blast resistance breeding for North Vietnam.
First two authors contributed equally |
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| Keywords: | AFLP genetic diversity Magnaporthe grisea Pyricularia grisea |
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