| 拟南芥动蛋白AtOvKLP的序列分析及原核表达 |
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| 引用本文： | 刘瑞娟,王海庆,沈裕虎.拟南芥动蛋白AtOvKLP的序列分析及原核表达[J].甘肃农业大学学报,2010,45(3). |
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| 作者姓名： | 刘瑞娟 王海庆 沈裕虎 |
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| 作者单位： | 中国科学院西北高原生物研究所,高原生物适应与进化重点实验室,青海,西宁,810001 |
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| 摘 要： | 选择拟南芥AtOvKLP蛋白作为研究对象,对其氨基酸序列和结构域进行了分析.结果表明,AtOvKLP蛋白ATP结合位点序列(FAYGQTGSGKT)与大多数动蛋白中的ATP结合位点序列不完全一致(YGQTGS-GKT);通过与动蛋白其它亚家族的系统分析比较,发现AtOvKLP与KHC亚家族类的各动蛋白距离较近;同时构建了pET-khc原核表达载体,并得到了体外表达蛋白.
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| 关 键 词： | 拟南芥 动蛋白 序列分析 原核表达 |
Sequence analysis and prokaryo expression of kinesin AtOvKLP in Arabidopsis thaliana |
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| LIU Rui-juan,WANG Hai-qing,SHEN Yu-hu.Sequence analysis and prokaryo expression of kinesin AtOvKLP in Arabidopsis thaliana[J].Journal of Gansu Agricultural University,2010,45(3). |
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| Authors: | LIU Rui-juan WANG Hai-qing SHEN Yu-hu |
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| Abstract: | AtOvKLP is one of kinesin-like proteins in Arabidopsis thaliana.The sequencing and motore region analysis displayed ATP-binding sequence of AtOvKLP (FAYGQTGSGKT)was different from other kinesins'(YGQTGSGKT). A close relationship between AtOvKLP and kinesins of KHC subfamily was supported by the phylogenetic analysis among AtOvKLP and other subfamilies. And expressed the AtOvKLP cDNA sequence in E.coil and obtained desired products in vitro for further study in validating the biochemistry activity of AtOvKLP. |
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| Keywords: | Arabidopsis thaliana kinesin sequence analysis prokaryo expression |
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