A standardised method for the quantitative analysis of resistance to grapevine powdery mildew |
| |
| Authors: | Anne-Sophie Miclot Sabine Wiedemann-Merdinoglu Eric Duchêne Didier Merdinoglu Pere Mestre |
| |
| Institution: | 1.INRA, UMR 1131 Santé de la Vigne et Qualité du Vin,Colmar,France;2.Université de Strasbourg, UMR 1131 Santé de la Vigne et Qualité du Vin,Colmar,France;3.UMR Génétique et Horticulture (GenHort), IFR149 QUASAV,Centre INRA Angers-Nantes,Beaucouzé,France;4.UMR 1131 Santé de la Vigne et Qualité du Vin,Colmar cedex,France |
| |
| Abstract: | Powdery mildew caused by Erysiphe necator is one of the most important diseases affecting grapevine (Vitis vinifera, L.). Control of this pathogen is based on the use of fungicides, which cause environmental damage and increase production
costs. A cost-effective and environmentally friendly alternative to control the disease relies on using resistant varieties.
While most V. vinifera cultivars are susceptible to powdery mildew, several species belonging to the Vitaceae have been described as resistant. Several loci for resistance to grapevine powdery mildew have been identified through genetic
analysis of segregating populations derived from different resistance sources. Identifying quantitative trait loci (QTL) with
minor effects on the resistance may prove valuable in a strategy of pyramiding, which aims at increasing the durability of
the resistance. However, current methods for evaluation of resistance either do not take into account quantitative variations,
or, if they do, are not adapted to large sample sets. Here we develop a method for the analysis of quantitative resistance
to grapevine powdery mildew in large populations. We devised a semi-quantitative resistance scale and confirmed the usefulness
of a cell counter to quantify sporulation. We compared three inoculation methods and identified dry inoculation using a settling
tower as the one giving the best infection. Finally, we confirmed the value of the method by applying it to a set of plants
segregating for resistance to E. necator. Using the method described here for the quantitative analysis of the resistance to powdery mildew will prove valuable for
breeding for durable resistance. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
