| 一株玉米弯孢叶斑病菌生长缓慢型突变株获得及T-DNA右翼序列分析 |
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| 引用本文： | 刘铜,薛文波,侯巨梅,左豫虎.一株玉米弯孢叶斑病菌生长缓慢型突变株获得及T-DNA右翼序列分析[J].黑龙江八一农垦大学学报,2011,23(3):4-8. |
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| 作者姓名： | 刘铜 薛文波 侯巨梅 左豫虎 |
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| 作者单位： | 黑龙江八一农垦大学农学院,大庆,163319 |
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| 摘 要： | 本试验采用农杆菌介导的遗传转化方法获得了1个菌落生长缓慢型的突变体,通过抗性基因(hph)的扩增及分子杂交验证了T-DNA以单拷贝的方式插入基因组中.对突变体的一些生物学性状与野生型CX-3比较,结果发现突变体的菌落生长速度是野生型CX-3的42%,其产孢量为野生弄CX-3的18%,但是对离体玉米叶片的致病力与野生型C...
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| 关 键 词： | 农杆菌 生长缓慢 T-DNA 致病力 右翼序列 |
Acquirement of a Slow-growth Mutant of Curvularia lunata and Analysis of the T-DNA Right Flanking Sequence |
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| Liu Tong,Xue Wenbo,Hou Jumei,Zuo Yuhu.Acquirement of a Slow-growth Mutant of Curvularia lunata and Analysis of the T-DNA Right Flanking Sequence[J].Journal of Heilongjiang August First Land Reclamation University,2011,23(3):4-8. |
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| Authors: | Liu Tong Xue Wenbo Hou Jumei Zuo Yuhu |
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| Institution: | Liu Tong,Xue Wenbo,Hou Jumei,Zuo Yuhu(College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319) |
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| Abstract: | A slow-growth mutant was obtained by Agrobacterium-Mediated transformation in this study.T-DNA as a single copy integrated into genome by using the method of resistant gene amplification and Southern hybridization.The biological characteristics of mutant were compared with the wild type stain.The results showed that the colony growth rate of the mutant was 42%,and sporulation was 18%,while there was no obvious difference in pathogenicity on detached maize between the two strains.The right flanking sequence ... |
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| Keywords: | T-DNA |
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