| 植物乳杆菌中胆盐水解酶结构基因的克隆与序列分析 |
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| 引用本文： | 李蓉,于长青.植物乳杆菌中胆盐水解酶结构基因的克隆与序列分析[J].黑龙江八一农垦大学学报,2011,23(3):55-57. |
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| 作者姓名： | 李蓉 于长青 |
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| 作者单位： | 黑龙江八一农垦大学食品学院,大庆,163319 |
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| 基金项目： | 大庆市高新区创新基金资助，校博士启动基金 |
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| 摘 要： | 胆盐水解酶主要是微生物在生长和繁殖过程中所产生的代谢产物;具有降低胆固醇的功能.克隆植物乳杆菌中的胆盐水解酶基因并对其序列进行分析.从植物乳杆菌中提取基因组DNA作为模板.根据Genbank上胆盐水解酶全长基因序列(EU477374)设计一对特异性引物,采用PCR扩增技术得到植物乳杆菌中胆盐水解酶基因并对其进行测序.结...
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| 关 键 词： | 植物乳杆菌 胆盐水解酶 克隆 |
Cloning and Sequence Analysis of BSH Gene of Lactobacillus Plantarum |
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| Li Rong,Yu Changqing.Cloning and Sequence Analysis of BSH Gene of Lactobacillus Plantarum[J].Journal of Heilongjiang August First Land Reclamation University,2011,23(3):55-57. |
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| Authors: | Li Rong Yu Changqing |
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| Institution: | (College of Foodstuff,Heilongjiang Bayi Agricultural University,Daqing163319) |
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| Abstract: | The BSH gene of Lactobacillus plantarum was cloned and sequence analyzed.Extract the genome DNA of Lactobacillus plantarum.According to the complete gene sequence of BSH in Genbank(EU477374),a pair of specific primer was designed,BSH gene of Lactobacillus plantrum were obtained by PCR amplify and predicted the sequence.Result indicate: The total length of cloned BSH gene was 995 bp;The homology between the predicted amino acid sequence and the EU477374 was 99.3%.Conclusion: BSH gene was successfully cloned which may lay the foundation for expression of BSH. |
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| Keywords: | lactobacillus plantarum BSH clone |
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