Anti-Inflammatory Components of the Starfish Astropecten polyacanthus |
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| Authors: | Nguyen Phuong Thao Nguyen Xuan Cuong Bui Thi Thuy Luyen Tran Hong Quang Tran Thi Hong Hanh Sohyun Kim Young-Sang Koh Nguyen Hoai Nam Phan Van Kiem Chau Van Minh Young Ho Kim |
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| Institution: | 1.Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi 10000, Vietnam; E-Mails: (N.P.T.); (N.X.C.); (B.T.T.L.); (T.H.Q.); (T.T.H.H.); (N.H.N.); (P.V.K.); (C.V.M.);2.College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea;3.School of Medicine, Brain Korea 21 Program, and Institute of Medical Science, Jeju National University, Jeju 690-756, Korea; E-Mails: (S.K.); (Y.-S.K.) |
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| Abstract: | Inflammation is important in biomedical research, because it plays a key role in inflammatory diseases including rheumatoid arthritis and other forms of arthritis, diabetes, heart disease, irritable bowel syndrome, Alzheimer’s disease, Parkinson’s disease, allergies, asthma, and even cancer. In the present study, we describe the inhibitory effect of crude extracts and steroids isolated from the starfish Astropecten polyacanthus on pro-inflammatory cytokine (Interleukin-12 (IL-12) p40, interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α)) production in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs). Among those tested, compounds 5 and 7 showed potent inhibitory effects on the production of all three pro-inflammatory cytokines with IC50 values ranging from 1.82 ± 0.11 to 7.00 ± 0.16 μM. Potent inhibitory activities were also observed for compound 1 on the production of IL-12 p40 and IL-6 with values of 3.96 ± 0.12 and 4.07 ± 0.13 μM, respectively, and for compounds 3 and 4 on the production of IL-12 p40 with values of 6.55 ± 0.18 and 5.06 ± 0.16 μM, respectively. Moreover, compounds 2 (IC50 = 34.86 ± 0.31 μM) and 6 (IC50 = 79.05 ± 2.05 μM) exhibited moderate inhibitory effects on the production of IL-12 p40, whereas compounds 3 (IC50 = 22.80 ± 0.21 μM) and 4 (IC50 = 16.73 ± 0.25 μM) moderately inhibited the production of TNF-α and IL-6, respectively. |
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| Keywords: | starfish Astropecten polyacanthus IL-12 p40 IL-6 TNF-α LPS-stimulated BMDCs |
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